Effects of Bupleurum chinense on the diversity of intestinal flora in depressed mice.
10.19540/j.cnki.cjcmm.20210524.703
- Author:
Sai-Bo CAI
1
;
Huan-Yu ZHOU
1
;
Xin-Yu JI
1
;
Qi-Lin ZHANG
1
;
Xian-Yu DENG
1
;
Feng WANG
1
;
Yong-Hui LI
2
;
Lian-Bing LIN
1
Author Information
1. Faculty of Life Science and Technology, Kunming University of Science and Technology Kunming 650500, China Engineering Research Center for Replacement Technology of Feed Antibiotics of Yunnan College Kunming 650500, China.
2. Key Laboratory of Mental Health, Chinese Academy of Sciences, Institute of Psychology, Chinese Academy of Sciences Beijing 100101, China Department of Psychology, University of Chinese Academy of Sciences Beijing 100049, China.
- Publication Type:Journal Article
- Keywords:
16S rDNA sequencing;
Bupleurum chinense;
depression;
intestinal flora
- MeSH:
Animals;
Bupleurum;
Feces;
Gastrointestinal Microbiome;
Lactobacillus;
Mice;
Mice, Inbred C57BL
- From:
China Journal of Chinese Materia Medica
2021;46(16):4222-4229
- CountryChina
- Language:Chinese
-
Abstract:
Bupleuri Radix, serving as the sovereign medicinal in many antidepressant compound preparations, has been proved effective in treating depression in mice, but its effect on the intestinal flora remains unclear. The present study aimed to investigate the effects of Bupleurum chinense(one of the original materials of Bupleuri Radix) on the behaviors and the diversity of intestinal flora of depressed mice. A depression mouse model was induced by repeated social defeat stress. Specifically, C57 BL/6 J male mice were exposed to the attack from the CD-1 mice. Then, C57 BL/6 J male mice were divided into a depression group and a B. chinense group, with normal saline and B. chinense administered(ig) respectively. Sucrose preference test and tail suspension test were conducted during and after the experiment respectively, to analyze the effects of B. chinense on the behaviors of the depressed mice. The feces were collected after the experiment. The V3-V4 16 S rDNA regions of intestinal flora of mice in each group were sequenced by Ion S5 TMXL for the analysis of the number of operational taxonomic units(OTUs), richness, alpha and beta diversity indexes, and differential phyla and genera. The results indicated that B. chinense could decrease depressive-like behaviors of mice, increase sucrose preference, and shorten the time of immobility in tail suspension test. After B. chinense intervention, the relative abundance of Firmicutes was significantly decreased, while that of Bacteroidetes was increased at the phylum level. At the genus level, the relative abundance of Lactobacillus and Lachnoclostridium decreased(P<0.05), while that of Bacteroides, Alistopes, etc. was elevated(P<0.05). The findings demonstrate that B. chinense can regulate the intestinal flora and improve the depressive-like behaviors of mice with depression.
