Qingre Xiaoyanning Capsules alleviate HSV-1 susceptibility induced by emotional stress.
10.19540/j.cnki.cjcmm.20210524.701
- Author:
Shu-Hua OUYANG
1
;
Xiao-Hua WANG
2
;
Wen LI
2
;
Zhuo LUO
2
;
Hong-Ying PENG
3
;
Tao JIANG
3
;
Yi-Fang LI
2
;
Hiroshi KURIHARA
2
;
Rong-Rong HE
4
Author Information
1. Guangdong Engineering Research Center of Chinese Medicine & Disease Susceptibility, Jinan University Guangzhou 510632,China School of Traditional Chinese Medicine,Jinan University Guangzhou 510632, China Integrated Chinese and Western Medicine Postdoctoral Research Station,Jinan University Guangzhou 510632, China.
2. Guangdong Engineering Research Center of Chinese Medicine & Disease Susceptibility, Jinan University Guangzhou 510632,China College of Pharmacy, Jinan University Guangzhou 510632, China.
3. Guangzhou Baiyunshan Jingxiutang Pharmaceutical Co., Ltd. Guangzhou 510130, China.
4. Guangdong Engineering Research Center of Chinese Medicine & Disease Susceptibility, Jinan University Guangzhou 510632,China College of Pharmacy, Jinan University Guangzhou 510632, China School of Traditional Chinese Medicine,Jinan University Guangzhou 510632, China Integrated Chinese and Western Medicine Postdoctoral Research Station,Jinan University Guangzhou 510632, China.
- Publication Type:Journal Article
- Keywords:
Qingre Xiaoyan-ning(QX) Capsules;
depression-induced liver fire;
emotional stress;
herpes simplex virus type 1(HSV-1);
susceptibility
- MeSH:
Animals;
Capsules;
Herpes Simplex;
Herpesvirus 1, Human;
Mice;
Mice, Inbred BALB C;
Psychological Distress
- From:
China Journal of Chinese Materia Medica
2021;46(16):4208-4213
- CountryChina
- Language:Chinese
-
Abstract:
In this study, emotional stress-induced herpes simplex virus type 1(HSV-1) susceptibility model was employed to simu-late the pathological state of " depression-induced liver fire", and the protection effect of Qingre Xiaoyanning(QX) in clearing liver fire was investigated. BALB/c mice were randomly divided into a normal group, a HSV-1 group, a restraint stress + HSV-1 group,low-(0. 658 g·kg~(-1)) and high-dose(1. 316 g·kg~(-1)) QX groups, and an acyclovir group. Except for the normal group and the HSV-1 group, the mice in other groups received daily restraint stress for 6 h from day 3 of medication. On day 9 of medication, mice were anesthetized by isoflurane and infected intranasally with HSV-1. Survival rate, weight change, encephalitis symptoms, and eye injury of mice were recorded for 14 d after virus infection. Hematoxylin-eosin(HE) staining and immunohistochemical staining were used to detect pathological changes and HSV-1 antigen distribution. Plaque assay was performed to detect the titer of HSV-1. The protein ex-pression of ICP27 in the mouse brain was detected by Western blot. The experimental results showed that QX could increase the survival rate of HSV-1-infected mice loaded with emotional stress(P<0. 001), reduce the titer of HSV-1 in the mouse brain(P<0. 01), relieve brain inflammation(P<0. 05) and eye injury(P<0. 05), down-regulate the expression of ICP27 related to HSV-1(P<0. 05), and decrease the distribution of HSV-1 antigen in the mouse brain. The results demonstrated that QX significantly reduced the susceptibility to HSV-1 induced by emotional stress, which is expected to provide a theoretical basis for the treatment and preven-tion of HSV-1 infection and promote the clinical development and application of Chinese medicine effective in clearing liver fire.
