Main components in butyl alcohol extract of Baitouweng Decoction inhibited neutrophil chemotaxis.
10.19540/j.cnki.cjcmm.20210521.702
- Author:
Yun YUN
1
;
Jing-Jing JIANG
1
;
Ya-Dong WANG
1
;
Zhi-Qing XU
1
;
Meng-Xiang ZHANG
1
;
Tian-Ming WANG
2
;
Jing SHAO
2
;
Chang-Zhong WANG
2
Author Information
1. School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine Hefei 230012, China Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine Hefei 230012, China.
2. School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine Hefei 230012, China Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine Hefei 230012, China Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui University of Chinese Medicine Hefei 230012, China.
- Publication Type:Journal Article
- Keywords:
F-actin;
PI3K/Akt signaling pathway;
aesculin;
anemoside B4;
berberine hydrochloride;
chemotaxis of neutrophils
- MeSH:
1-Butanol;
Berberine/pharmacology*;
Chemotaxis;
Drugs, Chinese Herbal/pharmacology*;
Neutrophils
- From:
China Journal of Chinese Materia Medica
2021;46(16):4201-4207
- CountryChina
- Language:Chinese
-
Abstract:
The present study aims to investigate the effects of the main components(aesculin, berberine hydrochloride, and anemoside B4) in the butyl alcohol extract of Baitouweng Decoction(BAEB) on the chemotaxis of neutrophils induced by dimethyl sulfoxide(DMSO). HL60 cells were cultivated in RPMI-1640 complete medium, and transferred into a 6-well plate(2 × 10~5 per mL) with 4 mL in each well, followed by incubation with DMSO at 1.3% for five days. The morphologic changes of cells were observed under an inverted microscope. The CD11 b expression after DMSO induction was analyzed by flow cytometry. The effects of aesculin, berberine hydrochloride, and anemoside B4 on the cell proliferation and migration were detected by CCK8 assay and Transwell assay, respectively. The effects of the main components on the production and polarization of F-actin protein were also examined by flow cytometry and laser confocal microscopy. PI3 K/Akt signaling pathway was checked by Western blot. As revealed by the results, neutrophil-like HL60 cells were observed after DMSO induction. The CD11 b expression in these cells increased significantly as indicated by the flow cytometry. Additionally, 100 μg·mL~(-1) aesculin, 8 μg·mL~(-1) berberine hydrochloride, and 80 μg·mL~(-1) anemoside B4 were potent in inhibiting the migration of neutrophils and reducing F-actin expression. Berberine hydrochloride was verified to be capable of diminishing phosphorylated PI3 K/Akt protein expression. The findings indicate that aesculin, anemoside B4, and especially berberine hydrochloride in the BAEB can inhibit the chemotaxis of neutrophils, which is possibly achieved by the inhibition of F-actin and PI3 K/Akt signaling pathway.
