Rapid chemome profiling of Cistanche salsa using DI-MS/MS~(ALL).
10.19540/j.cnki.cjcmm.20210331.202
- Author:
Li-Bo CAO
1
;
Xing-Cheng GONG
1
;
Jin-Ru JIA
1
;
Qian CAO
1
;
Peng-Fei TU
1
;
Qing-Qing SONG
1
;
Yue-Lin SONG
1
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100029, China.
- Publication Type:Journal Article
- Keywords:
Cistanche salsa;
DI-MS/MS~(ALL);
chemical constituents
- MeSH:
Chromatography, High Pressure Liquid;
Cistanche;
Drugs, Chinese Herbal;
Glycosides;
Plants, Medicinal;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(16):4150-4156
- CountryChina
- Language:Chinese
-
Abstract:
The current study aims to rapidly and comprehensively profile the chemical composition of Cistanche salsa using direct infusion coupled with MS/MS~(ALL)(DI-MS/MS~(ALL)). The C. salsa extract was directly imported into electrospray ionization(ESI) source of quadrupole time-of-flight(Q-TOF) mass spectrometer with an infusion pump at a flow rate of 10 μL·min~(-1). Acquisition program was applied under negative ionization polarity to collect one MS~1 spectrum(m/z 50-1 200), followed by 1 150 MS~2 spectra with precursor isolation window(m/z 1) amongst mass range m/z 50-1 200. After each MS~2 spectrum was matched to its precursor ion, putative identification was conducted through matching mass spectral data with literature and database. A total of 31 components were identified from C. salsa, including 9 phenylethanoid glycosides, 2 iridoids, 4 saccharides, 9 organic acids, and 7 other compounds, similar to those from C. tubulosa and C. deserticola. In conclusion, DI-MS/MS~(ALL), a facile and reliable analytical tool, can be employed for qualitative analysis of chemical constituents in C. salsa. The research offers a promising strategy to achieve rapid chemome profiling of herbal medicine and provides an alternative source of Cistanches Herba.
