Genetic diversity of protopine-6-hydroxylase in three medicinal Papaver plants.
10.19540/j.cnki.cjcmm.20210522.102
- Author:
Tian-Hua WU
1
;
Jun-Hui ZHOU
2
;
Yu-Yang ZHAO
2
;
Yu-Jie WEI
3
;
Fang CHEN
3
;
Yong-Fu GONG
3
;
Yuan YUAN
2
;
Lu-Qi HUANG
1
Author Information
1. Academician Workstation, Jiangxi University of Traditional Chinese Medicine Nanchang 330004, China National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Gansu Academy of Agri-Engineering Technology Wuwei 733000, China.
- Publication Type:Journal Article
- Keywords:
Papaver orientale;
Papaver rhoeas;
Papaver somniferum;
protopine-6-hydroxylase;
re-sequencing
- MeSH:
Benzophenanthridines;
Berberine Alkaloids;
Cytochrome P-450 Enzyme System/genetics*;
Genetic Variation;
Papaver/genetics*
- From:
China Journal of Chinese Materia Medica
2021;46(16):4111-4116
- CountryChina
- Language:Chinese
-
Abstract:
Sanguinarine is the main active component of the Papaver plants, and protopine-6-hydroxylase(P6 H), involved in the sanguinarine biosynthetic pathway, can oxidize protopine to 6-hydroxyprotopine. The investigation on the diversity of P6 H genes in the medicinal Papaver plants contributes to the acquirement of P6 H with high activity to increase the biosynthesis of sanguinarine. Five P6 H genes in P. somniferum, P. orientale, and P. rhoeas were discovered based on the re-sequencing data of the Papaver species, followed by bioinformatics analysis. With the elongation factor 1α(EF-1α), which exhibits stable expression in the root and stem, as the internal reference gene, the transcription levels of P6H genes in roots and stems of the Papaver plants were detected by real-time fluorescent quantitative PCR. As indicated by the re-sequencing results, there were two genotypes of P6H in P. somniferum and P. orientale, respectively, and only one in P. rhoeas. The bioinformatics analysis showed that the P6 H proteins of the three Papaver plants contained the conserved domain cl12078, which is the characteristic of p450 supergene family, and transmembrane regions. The existence of signal peptide remained verification. Real-time fluorescent quantitative PCR results revealed that the transcription level of P6 H in roots of P. somniferum was about 1.44 times of that in stems(α=0.05). The present study confirmed genetic diversity of P6 H in the three medicinal Papaver plants, which lays a basis for the research on the biosynthesis pathway and mechanism of sanguinarine in Papaver species.
