Study on potential effective components and mechanism of Zhishe Tongluo Capsules in treatment of ischemic stroke.
10.19540/j.cnki.cjcmm.20210305.402
- Author:
Yu LI
1
;
Ji-Xiang TIAN
2
;
He XU
2
;
Fang-Bo ZHANG
2
;
Hong-Jun YANG
3
Author Information
1. Tianjin University of Traditional Chinese Medicine Tianjin 301617, China Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Zhishe Tongluo Capsules;
ischemic stroke;
mechanism;
molecular docking;
network pharmacology
- MeSH:
Brain Ischemia/drug therapy*;
Capsules;
Drugs, Chinese Herbal;
Humans;
Ischemic Stroke;
Medicine, Chinese Traditional;
Molecular Docking Simulation;
Stroke/drug therapy*
- From:
China Journal of Chinese Materia Medica
2021;46(12):3024-3033
- CountryChina
- Language:Chinese
-
Abstract:
To explore the potential effective components and mechanism of Zhishe Tongluo Capsules in the treatment of ischemic stroke via network pharmacology, molecular docking and cellular experiment. The chemical constituents of Zhishe Tongluo Capsules were found by TCMSP, BATMAN-TCM and literatures. The constituents-target network was predicted by BATMAN-TCM database. Key words such as cerebral stroke, ischemic stroke and cerebral ischemic stroke were used to search ischemic stroke related targets, and then Venny Map was constructed based on the targets of traditional Chinese medicine and the targets of ischemic stroke. The overlapping targets were imported into STRING database to establish the interaction network. Furthermore, the core targets were screened out by Cytoscape software. Go and KEGG enrichment analysis were performed through DVIAD database. The results showed a total of 193 potential chemical constituents, 985 drug targets and 6 035 disease targets. There were 631 potential targets, 44 core targets and 55 potential active components for treating ischemic stroke through Venny mapping. GO enrichment analysis mainly involved response to hypoxia and positive regulation of ERK1/ERK2. KEGG pathway enrichment analysis mainly involved cholinergic synapse, cAMP signaling pathway, and calcium signaling pathway. Molecular docking data revealed that TP53, EGFR, IL6, INS, TNF and SRC had a good capability to bind with their corresponding active components. To ensure the protective effect Zhishe Tongluo Capsules on the inflammation reaction, an in vitro model of lipopolysaccharide(LPS)-induced RAW264.7 cells was built. The contents of IL-1α, IL-1β, IL-6 and TNF-α in the supernatant were significantly decreased by enzyme linked immunosorbent assay(ELISA). The findings suggested that Zhishe Tongluo Capsules could prevent the injury of ischemic stroke by inhibiting the inflammation.
