Qualitative and quantitative analysis of Paris polyphylla var. chinensis by UPLC-Q-TOF-MS/MS and HPLC.
10.19540/j.cnki.cjcmm.20210205.301
- Author:
Yan-Min LI
1
;
Liang-Jun GUAN
2
;
Liang-Mian CHEN
2
;
Meng ZHAO
2
;
Li-Shuai DING
2
;
Chen-Xiao-Ning MENG
2
;
Hui-Min GAO
2
;
Zhi-Min WANG
2
Author Information
1. National Engineering Laboratory of Quality Control Technology of Chinese Materia Medica, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China Beijing Health Vocational College Beijing 100053, China.
2. National Engineering Laboratory of Quality Control Technology of Chinese Materia Medica, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Paridis Rhizoma(PR);
Paris polyphylla var.chinensis;
chemical compositions;
non-medicinal parts;
steroidal saponins
- MeSH:
Chromatography, High Pressure Liquid;
Liliaceae;
Melanthiaceae;
Rhizome;
Saponins;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(12):2900-2911
- CountryChina
- Language:Chinese
-
Abstract:
Paridis Rhizoma(PR) is prepared from the dried rhizome of Paris polyphylla var. yunnanensis(PPY) or P. polyphylla var. chinensis(PPC) in Liliaceae family. The rapid development of PPY or PPC planting industry resulted from resource shortage has caused the waste of a large number of non-medicinal resources. To clarify the chemical compositions in rhizomes, fibrous roots, stems, leaves, seeds and pericarps of PPC, and explore the comprehensive application value and development prospect of these parts, the qualitative and quantitative analyses on the different parts of PPC were carried out by ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS) and high performance liquid chromatography(HPLC). A total of 136 compounds were identified, including 112 steroidal saponins, 6 flavonoids, 11 nitrogen-containing compounds and 7 phytosterols. Rhizomes, fibrous roots, and seeds mainly contained protopennogenyl glycosides and pennogenyl glycosides; leaves and stems mainly contained protodiosgenyl glycosides and diosgenyl glycosides; pericarps mainly contained pennogenyl glycosides, followed by diosgenyl glycosides. The total level of four saponins was the highest in fibrous roots and rhizomes, followed by those in the pericarps and arillate seeds, and the lowest in the stems and exarillate seeds. This study can provide data support for the comprehensive development and rational application of non-medicinal parts of PPC.
