Study on antidepressant effect of total alkaloids of Fibraurea recisa.
10.19540/j.cnki.cjcmm.20210406.403
- Author:
He YU
1
;
Zhong-Mei HE
1
;
Kun SHI
1
;
Yan ZHAO
1
;
Ying ZONG
1
;
Jian-Ming LI
1
;
Wei-Jia CHEN
1
;
Rui DU
2
Author Information
1. College of Chinese Medicinal Materials,Jilin Agricultural University Changchun 130118,China.
2. College of Chinese Medicinal Materials,Jilin Agricultural University Changchun 130118,China Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education of China Changchun 130118,China Jilin Provincial Engineering Research Center for Efficient Breeding and Product Development of Sika Deer of China Changchun 130118,China.
- Publication Type:Randomized Controlled Trial, Veterinary
- Keywords:
CUMS mice;
HT22 cells;
depression;
total alkaloids of Fibraurea recisa
- MeSH:
Alkaloids/pharmacology*;
Animals;
Antidepressive Agents/pharmacology*;
Depression/drug therapy*;
Disease Models, Animal;
Hippocampus;
Mice;
Mice, Inbred C57BL;
Stress, Psychological
- From:
China Journal of Chinese Materia Medica
2021;46(14):3678-3686
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to investigate the antidepressant effects of total alkaloids of Fibraurea recisa in HT22 cells damaged by corticosterone (CORT) in vitro and in a mouse model of chronic unpredictable mild stress (CUMS) as well as the underlying mechanisms.In cellular experiments,the viability of CORT-damaged HT22 cells was detected using cell counting kit-8 (CCK-8),and the cell apoptosis was detected by Hoechst 33258 staining.In animal experiments,C57BL/6N mice were randomly divided into the control group,model group,low (100 mg·kg~(-1)),medium (200 mg·kg~(-1)) and high (400 mg·kg~(-1))-dose of total alkaloids of F.recisa groups,and positive control group.After 21 days of CUMS exposure,their depressive behaviors were observed in behavioral and Morris water maze tests.The serum levels of 5-hydroxytryptamine (5-HT),dopamine (DA),and norepinephrine (NE) were assessed by ELISA.The expression levels of apoptosis-related proteins Bcl-2,Bax and cleaved caspase-3 in HT22 cells and mouse hippocampus were detected by Western blot.The results suggested that total alkaloids of F.recisa alleviated the damage of HT22 cells induced by CORT in a dose-dependent manner.The Hoechst 33258 staining uncovered that total alkaloids of F.recisa better reduced the blue spots and inhibited cell apoptosis.The results of animal experiments showed that total alkaloids of F.recisa significantly improved the depression-like behaviors of mice and increased the serum levels of 5-HT,DA and NE as compared with those in the model group.The Western blot assays revealed a significant up-regulation of Bcl-2 protein expression,but an obvious reduction in Bax and cleaved caspase-3protein expression in the total alkaloids of F.recisa group.In conclusion,total alkaloids of F.recisa inhibited depression possibly by regulating the apoptosis-related protein expression or elevating the monoamine neurotransmitter levels in the brain.
