Molecular identification and alkaloids content of endangered Chinese medicinal plant Coptis chinensis var. brevisepala.
10.19540/j.cnki.cjcmm.20210430.102
- Author:
An-Qi WANG
1
;
Xi-Wen LI
1
;
Qing-Jun YUAN
2
;
Ning GUO
1
;
Bin YANG
1
;
Yi SUN
1
Author Information
1. Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
2. National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
Coptis chinensis var.brevisepala;
UPLC-QTOF-MS;
isoquinoline alkaloids;
molecular identification;
quantitative analysis
- MeSH:
Alkaloids;
Berberine Alkaloids;
China;
Coptis;
Drugs, Chinese Herbal/analysis*;
Plants, Medicinal
- From:
China Journal of Chinese Materia Medica
2021;46(14):3514-3521
- CountryChina
- Language:Chinese
-
Abstract:
According to the records of Chinese materia medica,Coptis chinensis var. brevisepala is an authentic Chinese medicinal plant highly recommended by ancient physicians since its rhizome is like a string of beads and has a good medicinal value. However,its medicinal components and values remain to be studied as it is endangered because of overexploitation. Therefore,this study aims to quantitatively determine its effective components based on UPLC-QTOF-MS,and to compare the contents of isoquinoline alkaloids in C.chinensis var. brevisepala with those in other Coptis species. Meanwhile,molecular methods accurately identified 12 batches of C. chinensis var. brevisepala,9 batches of C. chinensis,4 batches of C. deltoidea,and 1 batch of C. teeta. Gradient elution was performed with Waters CORTECS C18 column( 4. 6 mm× 150 mm,2. 7 μm) and the mobile phase acetonitrile-water with 0. 4% formic acid. Mass spectrometry was conducted in ESI positive mode. The quantitative results showed that 8 main alkaloids had a good linear relationship within the concentration range( R~2>0. 996),with the recovery rate of 95. 18%-105. 0% and the RSD of 0. 28%-3. 7%. Compared with that of other Coptis species,the rhizome of C. chinensis var. brevisepala had the highest contents of berberine and columbamine. The total content of the 8 alkaloids in C. chinensis var. brevisepala was similar to that in C. chinensis but higher than that of the other two species. PCA was performed to compare the alkaloids among the 4 species. Besides,the 8 alkaloids were evaluated in different parts of C. chinensis var. brevisepala. The results indicate that this method is reliable and efficient and can provide a reference for the quality research.
