Identification,biological characteristics and fungicide screening of pathogen of southern blight in Cynanchum stauntonii.
10.19540/j.cnki.cjcmm.20210318.101
- Author:
Jin-Xin LI
1
;
Qiao-Huan CHEN
1
;
Yu-Huan MIAO
1
;
Tie-Lin WANG
2
;
Da-Hui LIU
1
Author Information
1. Pharmacy Faculty,Hubei University of Chinese Medicine Wuhan 430065,China.
2. State Key Laboratory Breeding Base of Dao-di Herbs,National Resouce Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
- Publication Type:Journal Article
- Keywords:
Athelia rolfsii;
Cynanchum stauntonii;
biological characteristics;
fungicide screening;
southern blight
- MeSH:
Basidiomycota;
Cynanchum;
Fungicides, Industrial/pharmacology*;
Humidity
- From:
China Journal of Chinese Materia Medica
2021;46(13):3303-3310
- CountryChina
- Language:Chinese
-
Abstract:
During the high-temperature and rainy season from June to October in 2017-2019,serious southern blight broke out in the Cynanchum stauntonii planting area in Tuanfeng county,Hubei province,which had a great impact on the yield and quality of medicinal materials. In this study,the pathogen of C. stauntonii was isolated,purified,and identified,and the pathogenicity was tested according to Koch's postulates. Meanwhile,the biological characteristics of the pathogen were analyzed. On this basis,the effective fungicides were screened in laboratory. Finally,the pathogen( BQ-1) was identified as Athelia rolfsii( Deuteromycotina,Basidiomycota,anamorph: Sclerotium rolfsii). The optimum growth conditions for BQ-1 were 25-30 ℃,p H 5-8,and alternating light and dark.The effective chemical fungicides were lime-sulphur-synthelic-solution( LSSS) and flusilazole,and the effective botanical fungicide was osthole. BQ-1 was highly homologous to the pathogen HS-1 of peanut southern blight,with the similarity of 18 S r DNA and TEF sequences at 99. 09%. The southern blight in C. stauntonii might be resulted from that in peanut. In the production of C. stauntonii,the following measures should be taken: avoiding rotation or neighboring with peanut,draining water from June to October to reduce humidity,and reasonably applying fungicides.
