Research progress on in vitro models of cardiomyocyte injury.
10.19540/j.cnki.cjcmm.20210311.601
- Author:
Yan-Yi LI
1
;
Jun-Jun LI
1
;
Fu-Xing GE
1
;
Xiao-Jing MA
1
;
Chun LI
1
;
Xiao-Ni AI
2
;
Xiao-Li GAO
1
;
Peng-Fei TU
3
;
Xing-Yun CHAI
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029,China.
2. School of Pharmaceutical Sciences,Peking University Health Science Center Beijing 100083,China.
3. Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029,China School of Pharmaceutical Sciences,Peking University Health Science Center Beijing 100083,China.
- Publication Type:Journal Article
- Keywords:
heart disease;
injury model;
myocardial cell;
myocardial injury;
review
- MeSH:
Apoptosis;
Cell Hypoxia;
Humans;
Myocardium;
Myocytes, Cardiac;
Oxidative Stress
- From:
China Journal of Chinese Materia Medica
2021;46(13):3257-3269
- CountryChina
- Language:Chinese
-
Abstract:
Cardiovascular diseases seriously endanger human health and life. The accompanying myocardial injury has been a focus of attention in society. Chinese medicine,serving as a natural and precious reservoir for the research and development of new drugs,is advantageous in resisting myocardial injury due to its multi-component,multi-pathway,and multi-target characteristics. In recent years,with the extensive application of culture method for isolated cardiomyocytes,a cost-effective,controllable in vitro model of cardiomyocyte injury with uniform samples is becoming a key tool for mechanism research on cardiomyocyte injury and drug development.A good in vitro model can reduce experimental and manpower cost,and also accurately stimulate clinical changes to reveal the mechanism. Therefore,the selection and establishment of in vitro model are crucial for the in-depth research. This study summarized the modeling principles,evaluation indicators,and application of more than ten models reflecting different clinical conditions,such as injuries induced by hypoxia-reoxygenation,hypertrophy,oxidative stress,inflammation,internal environmental disturbance,and toxicity. Furthermore,we analyzed advantages and technical difficulties,aiming to provide a reference for in-depth research on myocardial injury mechanism and drug development.
