Changes in forms of Indigo Naturalis slices in ancient and modern times: a systematic study on quality of purified Indigo Naturalis and crude Indigo Naturalis.
10.19540/j.cnki.cjcmm.20210408.301
- Author:
Le-le MA
1
;
Run-Chun XU
1
;
Ding-Kun ZHANG
1
;
Li-Ming CHEN
2
;
Yi WU
3
;
Hao-Zhou HUANG
1
;
Fang WANG
3
;
Jun-Zhi LIN
4
;
Li HAN
1
;
Ming YANG
3
Author Information
1. State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine Chengdu 611137,China.
2. Sichuan Lianchengxunkang Medical & Pharmaceutical Co.,Ltd. Yaan 625000,China.
3. Key Laboratory of Modern Chinese Medicine Preparation of Ministry of Education,Jiangxi University of Traditional Chinese Medicine Nanchang 330004,China.
4. Traditional Chinese Medicine Regulating Metabolic Diseases Key Laboratory of Sichuan Province,Hospital of Chengdu University of Traditional Chinese Medicine Chengdu 610072,China.
- Publication Type:Journal Article
- Keywords:
Indigo Naturalis;
antipyretic;
crude Indigo Naturalis;
processing technology;
purified Indigo Naturalis;
quality comparison
- MeSH:
Animals;
Indigo Carmine;
Indigofera;
Plant Extracts;
Rats;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(13):3188-3197
- CountryChina
- Language:Chinese
-
Abstract:
Indigo Naturalis has a long history of medicinal use with particularity and complexity in its processing. Before the Ming dynasty,Indigo Naturalis was extracted from the top layer of zymotic fluid,called " purified Indigo Naturalis". In modern processing,the precipitate " crude Indigo Naturalis" is dried to produce Indigo Naturalis after impurity removal. The form of Indigo Naturalis slices has undergone significant changes in ancient and modern times. In view of this,the quality comparison between crude Indigo Naturalis and purified Indigo Naturalis was conducted in this study with modern analytical techniques. Firstly,chemical composition was analyzed with UPLC-Q-TOF-MS,and the chemical composition of scent with HS-SPME/GC-MS/MS. The content of indigo,indirubin,total ash,and water-soluble extract was determined as well as the inorganic composition in crude Indigo Naturalis and purified Indigo Naturalis. Then,their microscopic morphology was observed and the surface element composition was investigated. Finally,the antipyretic activities of crude Indigo Naturalis and purified Indigo Naturalis were compared in the fever rat model induced by lipopolysaccharide and 2,4-dinitrophenol. The results demonstrated that the purified Indigo Naturalis had a faster and more lasting antipyretic effect,while the crude Indigo Naturalis had almost no antipyretic effect. This study is of great significance to the research on processing technology of Indigo Naturalis and provides reference for the formulation of its quality standards,production specifications and calibration procedures.
