Quantitation and content investigation of bilirubin in cultured cow-bezoar.
10.19540/j.cnki.cjcmm.20210305.202
- Author:
Yan SHI
1
;
Feng WEI
1
;
Bao-Jun LYU
2
;
Yan-Fang JIN
2
;
Nan CHEN
2
;
Fang-Fang DING
2
;
Shuang-Cheng MA
1
Author Information
1. National Institutes for Food and Drug Control Beijing 102629, China.
2. Shang Pharmacy (Ningxia) Pharmaceutical Co., Ltd. Yinchuan 750002, China.
- Publication Type:Journal Article
- Keywords:
HPLC;
bilirubin;
content determination;
cow-bezoar;
cultured cow-bezoar
- MeSH:
Animals;
Bezoars;
Bilirubin;
Cattle;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Female
- From:
China Journal of Chinese Materia Medica
2021;46(11):2852-2856
- CountryChina
- Language:Chinese
-
Abstract:
A determination method for bilirubin in cultured cow-bezoar was developed in this study, with which the bilirubin in 15 batches of samples was quantified. The samples were first processed with 10% oxalic acid solution for the conversion of bilirubin from conjugated to unconjugated, followed by the extraction with dichloromethane. Then the obtained sample solutions were analyzed at 450 nm by HPLC[chromatographic column: Agilent TC-C_(18)(4.6 mm × 250 mm, 5 μm); mobile phase: acetonitrile and 1% glacial acetic acid aqueous solution(95∶5); flow rate: 1.0 mL·min~(-1)]. The bilirubin content in the 15 batches of cultured cow-bezoar was ranged from 21.9% to 41.7% with the average of 32.4%. The proposed method is accurate and reliable, thus making it suitable for the quantitation of bilirubin in cultured cow-bezoar and its quality assessment and control.
