Predictive analysis of quality markers of Coptidis Rhizoma based on network pharmacology and multivariate statistical analysis.
10.19540/j.cnki.cjcmm.20210125.302
- Author:
Chun-Xia LI
1
;
Qiao-Yu HE
1
;
Jing LIU
1
;
Yu-Cheng WANG
1
;
Bei-Bei XUE
1
;
Qian TANG
1
;
Hong LIU
1
;
Er-Wei LIU
1
;
Xiao-Peng CHEN
1
Author Information
1. State Key Laboratory of Component-based Chinese Medicine,Tianjin University of Traditional Chinese Medicine Tianjin 301617,China.
- Publication Type:Review
- Keywords:
Coptidis Rhizoma;
fingerprint;
multivariate statistical analysis;
network pharmacology;
quality marker
- MeSH:
Alkaloids;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Multivariate Analysis;
Rhizome
- From:
China Journal of Chinese Materia Medica
2021;46(11):2718-2727
- CountryChina
- Language:Chinese
-
Abstract:
Coptidis Rhizoma, as a bulk medicinal material, is in great demand in clinical practice. Its quality is uneven in the market due to the mixture of genuine, counterfeit and adulterants. Therefore, it is particularly important to establish a quality control system for Coptidis Rhizoma. Based on the concept of Chinese medicine quality marker(Q-marker), the potential quality markers of Coptidis Rhizoma were analyzed and predicted from the perspective of chemistry and pharmacology. The sources of the Q-markers of Coptidis Rhizoma were identified by literature retrieval. The potential Q-markers were then screened through the visualization of the "components-targets-pathways" network. High performance liquid chromatography(HPLC) was used to establish a multi-indicator qualitative and quantitative control method featuring fingerprints for 10 batches of Coptidis Rhizoma. A supervised mode of orthogonality partial least squares method-discriminant analysis(OPLS-DA) was used to screen the main marker components that caused differences between groups. The literature review results showed that the alkaloids were the main source of Coptidis Rhizoma Q-markers.The fingerprints of 13 common peaks were successfully established, and berberine, palmatine, berberine and epiberberine were selected as Q-markers of Coptidis Rhizoma, and their contents were determined.Based on the concept of the Q-marker of traditional Chinese medicine, the four components can be selected as the Q-marker of Coptidis Rhizoma after comprehensive consideration. The results of this study are not only conducive to the quality evaluation of Coptidis Rhizoma on the market, but also provide a reference for the overall quality control of Coptidis Rhizoma and lay foundation for the future exploration of the mechanism of Coptidis Rhizoma.
