Explore antioxidant quality markers of Hippophae tibetana based on "dry-method + wet-method" technology.
10.19540/j.cnki.cjcmm.20210122.301
- Author:
Jin-Chai QI
1
;
Jing CHEN
2
;
Wen LI
1
;
Guo-Ping LI
1
;
Hong-Shan CHEN
1
;
Wen-Min PI
1
;
Feng GAO
1
;
Peng-Long WANG
1
;
Ma MI
2
;
Hai-Min LEI
1
Author Information
1. School of Traditional Chinese Pharmacy,Beijing University of Chinese Medicine Beijing 102488,China.
2. Tibetan Traditional Medical College Lhasa 850000,China.
- Publication Type:Journal Article
- Keywords:
Hippophae tibetana;
UPLC-Q-Exactive Orbitrap-MS;
anti-oxidation;
network pharmacology;
quality marker
- MeSH:
Antioxidants;
Chromatography, High Pressure Liquid;
Hippophae;
Mass Spectrometry;
Technology
- From:
China Journal of Chinese Materia Medica
2021;46(11):2699-2709
- CountryChina
- Language:Chinese
-
Abstract:
The cross combination of dry-method(network pharmacology analysis) and wet-method(high-resolution mass spectro-metry with antioxidation experiment) was used to predict antioxidant quality markers(Q-markers) of Hippophae tibetana. Ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS) was developed to rapidly separate and identify the chemical constituents in H. tibetana. Then in DPPH free radicals and superoxide anion scavenging experiment, the antioxidant activity of the four different polar parts with extracts of petroleumether, ethyl acetate, n-butanol and water was evaluated. Network pharmacology method was used for functional enrichment and pathway analysis to screen antioxidant-related components and preliminarily explain the mechanism of action. On this basis, multi-source information was integrated to predict the antioxidant Q-markers. The results showed that 51 components in H. tibetana were identified, including 18 flavonoids, 14 terpenoids, 6 alkaloids, 4 coumarins and phenylpropanoids, 3 volatile components and 2 polyphenols. The antioxidant capacity of different fractions: ethyl acetate > n-butanol > water > petroleum ether. The medicine mainly acted on PI3 K-Akt and FoxO signaling pathways to perform antioxidant effects through flavonoids such as quercetin, luteolin and kaempferol. According to the results of dry-method and wet-method, quercetin, luteolin and kaempferol, the representatives of poly-hydroxy flavone, may be the antioxidant Q-markers of H. tibetana. In this study, with the antioxidant Q-markers of H. tibetana as an example, an investigation model of predicting Q-marker was discussed based on the ternary system of composition, function and informatics, providing a scientific basis for the establishment of quality evaluation standards for H. tibetana.
