Feasibility of Preheating at 41 ℃ to Correct Red Blood Cell Parameters in the Presence of High-titer Cold Agglutinins.
10.3881/j.issn.1000-503X.13746
- Author:
Hong-Xiang XIE
1
;
Yan SHEN
1
;
Zhen-Zhen PANG
1
;
Bin SUN
1
;
Zhen-Ni WANG
1
;
Shu-Hui XIE
1
;
Yong-Lie ZHOU
1
Author Information
1. Center of Laboratory Medicine,Zhejiang Provincial Peoples' Hospital,People's Hospital of Hangzhou Medical College,Hangzhou 310014,China.
- Publication Type:Journal Article
- Keywords:
cold agglutinins;
complete blood count;
red blood cell parameters
- MeSH:
Cryoglobulins;
Erythrocyte Count;
Erythrocytes;
Feasibility Studies;
Hematocrit
- From:
Acta Academiae Medicinae Sinicae
2021;43(4):507-512
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the feasibility of preheating in 41 ℃ water bath for 30 minutes to correct the red blood cell parameters in the specimens containing high-titer cold agglutinins(CAs). Methods Two specimens containing high-titer CAs were selected during work,and the parameters of complete blood count at room temperature or after preheating in 37 ℃ or 41 ℃ water bath were compared.The smears were stained,and the distribution of red blood cells was observed with a microscope.Further,74 specimens without CAs were collected for complete blood count,and then the test results at room temperature and after preheating at 41 ℃ were compared. Results At room temperature,the specimens containing high-titer CAs showed significantly reduced red blood cell count(RBC)and hematocrit(HCT),abnormally increased mean corpuscular hemoglobin(MCH)and mean cell hemoglobin concentration(MCHC),abnormal percents of hemoglobin(HGB)and RBC,and aggregation of a large number of red blood cells.After being preheated at 37 ℃ for a certain time,the specimens demonstrated obviously improved parameters while still aggregation of a small number of red blood cells.After being preheated at 41 ℃ for 30 minutes,the specimens showed significantly increased RBC,normal HCT,MCH,and MCHC,and evenly distributed red blood cells.The 74 specimens without CAs showed the comparability was ≥80% between room temperature and preheating at 41 ℃ for 30 minutes or 60 minutes. Conclusion We can preheat the specimens containing high-titer CAs in a water bath at 41 ℃ to obtain accurate red blood cell parameters.
