In silico cloning, expression and bioinformatics analysis of NtODB from common tobacco.

Tao Chen; Xinyi Peng; Jianfeng Qin; Xu Qin; Mi WU; Jinyuan Huang; Xianya Huang; Danni Wei; Liping Wang; Gang Jin

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In silico cloning, expression and bioinformatics analysis of NtODB from common tobacco.

Author: Tao CHEN ¹ ; Xinyi PENG ¹ ; Jianfeng QIN ¹ ; Xu QIN ¹ ; Mi WU ¹ ; Jinyuan HUANG ¹ ; Xianya HUANG ¹ ; Danni WEI ¹ ; Liping WANG ¹ ; Gang JIN ¹
Author Information

1. Guangxi Subtropical Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530001, Guangxi, China.
Publication Type:Journal Article
Keywords: DNA repair; NtODB; common tobacco; in silico cloning
MeSH: Amino Acid Sequence; Base Sequence; Cloning, Molecular; Computational Biology; Computer Simulation; DNA, Complementary; Phylogeny; Plant Breeding; Tobacco/genetics*
From: Chinese Journal of Biotechnology 2021;37(8):2836-2844
CountryChina
Language:Chinese
Abstract: It has been reported that ODB genes play an important role in homologous recombination-directed DNA repair, suggesting their potential applications in plant breeding. To analyze the expression characteristics of tobacco NtODB gene, the cDNA sequence of NtODB was obtained using in silico cloning technique. The physicochemical properties, signal peptide, and advanced structures of the predicted protein were analyzed using bioinformatics tools. The results showed that the NtODB gene has a 579-bp open reading frame which encodes a protein with 192 amino acid residues. The protein NtODB is predicted to be alkaline and hydrophilic. Real-time quantitative PCR showed that NtODB was constitutively expressed in different tissues. Subcellular localization showed that NtODB was mainly expressed in cell membrane and chloroplast. These results may help us to better understand and elucidate the roles of ODB genes in the homologous recombination-directed DNA repair.