Construction of tissue engineered cell sheet.

Fuan Xiao; Xueting Jian; Xiaoyi Feng; Junyan Tan; Wanling Xiong; Ye Zhi; Yuan XU; Yang Liu

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Construction of tissue engineered cell sheet.

Author: Fuan XIAO ¹ ; Xueting JIAN ¹ ; Xiaoyi FENG ¹ ; Junyan TAN ¹ ; Wanling XIONG ¹ ; Ye ZHI ¹ ; Yuan XU ¹ ; Yang LIU ¹
Author Information

1. Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, College of Pharmacy, University of South China, Hengyang 421001, Hunan, China.
Publication Type:Review
Keywords: cell adhesion; cell detachment; cell sheet; sensitive materials; tissue engineering
MeSH: Temperature; Tissue Engineering; Tissue Scaffolds
From: Chinese Journal of Biotechnology 2021;37(7):2405-2413
CountryChina
Language:Chinese
Abstract: Scaffold-free tissue engineered cell sheet is an emerging technology in biomedical field. It can avoid the adverse effects of scaffold materials, and can be further assembled to form more complex three-dimensional functional tissues. The construction of cell sheet is mainly based on the culture substrate composed of sensitive materials. By changing the stimulation factors such as temperature, enzyme, light, ion, redox, pH and sugar, the adhesion behavior of the substrate to the cells could be changed to make the cells detach naturally, thus generating the cell sheet. Recent years have seen the development of various simple and efficient construction technologies of cell sheet due to the development of a variety of novel sensitive culture substrates. The resulted cell sheets with excellent performance have greatly expanded their applications. This review summarized the construction methods of tissue engineered cell sheet and discussed the challenges and future perspectives in this field.