Advances and application of enrichment technology in SH2 superbinder-based tyrosine phosphoproteomics.

Author: Liying MEN ¹ ; Feng XU ² ; Ping XU ¹
Author Information

1. School of Basic Medicine, Anhui Medical University, Hefei 230032, Anhui, China.
2. State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Beijing Institute of Lifeomics, Academy of Military Medical Sciences of Academy of Military Science, Beijing 102206, China.
Publication Type:Review
Keywords: SH2 superbinder; enrichment technology; membrane protein; micro-sample; tyrosine phosphorylation
MeSH: Cell Movement; Peptides; Phosphorylation; Technology; Tyrosine/metabolism*
From: Chinese Journal of Biotechnology 2021;37(7):2334-2341
CountryChina
Language:Chinese
Abstract: Tyrosine phosphorylation is one of the important protein phosphorylations in eukaryotes responsible for a variety of biological processes including cell signaling transduction, cell migration, and apoptosis. In the study of phosphoproteomics, due to the low stoichiometry of tyrosine phosphorylation (pTyr) proteins and sometimes limited initial sample, traditional phosphoproteomics enrichment technology is inefficient for the enrichment of pTyr peptides. Here, we review the substantial progress in tyrosine phosphoproteomics by preparation of limited amount sample and the newly introduced SH2 superbinder.