Research progress in the rapid preparation of monoclonal antibodies of mouse hybridoma.

Author: Shuiqin FANG ¹ ; Cheng LIU ¹ ; Junfei MA ¹ ; Shuaishuai YAN ¹ ; Dongpo XU ¹ ; Qing LIU ¹
Author Information

1. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
Publication Type:Journal Article
Keywords: antigen-specific; monoclonal antibody; mouse hybridoma; rapid preparation
MeSH: Animals; Antibodies, Monoclonal; Antigens; B-Lymphocytes; Hybridomas; Mice
From: Chinese Journal of Biotechnology 2021;37(7):2293-2306
CountryChina
Language:Chinese
Abstract: Mouse hybridoma monoclonal antibody is the most commonly used antibody in immunology because of its stable source, easy preparation in later stage and high yield. The traditional time-consuming and laborious hybridoma preparation technology could not meet the growing market demand. In this paper, we describe the rapid preparation techniques involved in antigen design and screening, B cell enrichment and screening, transgenic myeloma cells, fusion technology improvement, positive hybridoma cell screening and rapid detection of monoclonal antibody performance, to provide a reference for rapid preparation of mouse hybridoma monoclonal antibody.