Advances in quantification of lentiviral vectors.

Siqi Chen; Songping Zhang; Yanli Yang; Heping LI; Yu Wang; Youyan Liu

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Advances in quantification of lentiviral vectors.

Author: Siqi CHEN ¹ ; Songping ZHANG ² ; Yanli YANG ² ; Heping LI ³ ; Yu WANG ³ ; Youyan LIU ¹
Author Information

1. College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530000, Guangxi, China.
2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
3. Beijing YongtaiRuike Biotechnology Co. Ltd., Beijing 100176, China.
Publication Type:Journal Article
Keywords: infective titer; lentiviral vectors; particle analysis; quantification
MeSH: Genetic Vectors/genetics*; Humans; Immunotherapy; Lentivirus/genetics*; Neoplasms; Transduction, Genetic
From: Chinese Journal of Biotechnology 2021;37(7):2283-2292
CountryChina
Language:Chinese
Abstract: Immunotherapy is becoming an effective and less invasive strategy that can be applied to the treatment of various malignancies. Lentiviral vectors (LVs) have shown great potential in immunotherapy as they can stably integrate relatively large foreign DNA, and effectively transduce dividing and non-dividing cells. Clinical application needs high quality LVs, and therefore strict quality control of the final products is necessary to ensure their purity, efficacy and safety. The quantitative detection of LVs is among the key parts of product development and quality control. In this paper, the existing methods for quantitative detection of LVs are summarized, including fluorescence activated cell sorter (FACS), P24 enzyme-linked immuno sorbent assay (P24 ELISA), real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), nanoparticle tracking analysis (NTA), tunable resistive pulse sensing(TRPS) and virus counter(VC).Their advantages and disadvantages are listed, and future development and challenges are discussed.