Distribution of bacteria infected by metagenomic sequencing technology in maxillofacial space.
- Author:
Yi-Heng CHEN
1
;
Hong-Yu ZHENG
1
;
Zi-Xuan LI
1
;
Yong-Chao WU
1
;
Zhi-Xing NIU
1
;
Yan-Hui PENG
1
;
Jun-Fang ZHAO
1
;
Qiang SUN
1
Author Information
- Publication Type:Journal Article
- Keywords: maxillofacial space infection; metagenomic next-generation sequencing; next-generation sequencing technology; pathogenic microorga-nism
- MeSH: Bacteria/genetics*; High-Throughput Nucleotide Sequencing; Humans; Metagenomics; Sensitivity and Specificity; Technology
- From: West China Journal of Stomatology 2021;39(4):475-481
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:This study aimed to compare and analyze the consistency and difference between metageno-mic next-generation sequencing (mNGS) and conventional bacterial culture in the detection of pathogenic microorganisms in maxillofacial space infection, as well as to provide a new detection method for the early clinical identification of pathogenic bacteria in maxillofacial space infection.
METHODS:The clinical data of 16 patients with oral and maxillofacial space infections in the First Affiliated Hospital of Zhengzhou University from March 2020 to June 2020 were collected. mNGS and conventional bacterial culture methods were used to detect pus. We then analyzed and compared the test results of the two methods, including the test cycle, positive detection rate, anaerobic bacteria, facultative anaerobes and aerobic bacteria detection rates, distribution of pathogenic bacteria, relative species abundance, and resistance genes.
RESULTS:The average inspection period of mNGS was (18.81±3.73) h, and the average inspection period of bacterial culture was (83.25±11.64) h, the former was shorter than the latter (
CONCLUSIONS:Compared with conventional bacterial culture, mNGS has the characteristics of short test time, high sensitivity, and high accuracy. Thus, it is a new detection method for the early identification of pathogenic bacteria in maxillofacial space infection and is beneficial to the early clinical diagnosis and treatment of the disease.
