Regulation effect of lipopolysaccharide on the alternative splicing and function of sweet taste receptor T1R2.
- Author:
Jian-Hui ZHU
1
;
Xin ZHENG
1
;
Xian PENG
1
;
Xin XU
1
;
Robert MARGOLSKEE
2
;
Xue-Dong ZHOU
1
Author Information
- Publication Type:Journal Article
- Keywords: alternative splicing; lipopolysaccharide; sweet taste receptor; taste perception
- MeSH: Alternative Splicing; Animals; Lipopolysaccharides; Mice; Receptors, G-Protein-Coupled/metabolism*; Taste; Taste Buds
- From: West China Journal of Stomatology 2021;39(4):469-474
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To identify the alternative splicing isoform of mouse sweet taste receptor T1R2, and investigate the effect of lipopolysaccharide (LPS) local injection on T1R2 alternative splicing and the function of sweet taste receptor as one of the bacterial virulence factors.
METHODS:After mouse taste bud tissue isolation was conducted, RNA extraction and reverse transcription polymerase chain reaction (PCR) were performed to identify the splicing isoform of T1R2. Heterologous expression experiments
RESULTS:T1R2 splicing isoform T1R2_Δe3p formed sweet taste receptors with T1R3, which could not be activated by sweet taste stimuli and significantly downregulated the function of canonical T1R2/T1R3. Local LPS injection significantly increased the expression ratio of T1R2_Δe3p in mouse taste buds.
CONCLUSIONS:LPS stimulation affects the alternative splicing of mouse sweet taste receptor T1R2 and significantly upregulates the expression of non-functional isoform T1R2_Δe3p, suggesting that T1R2 alternative splicing regulation may be one of the mechanisms by which microbial infection affects host taste perception.
