circ_0005379 inhibits the progression of oral squamous cell carcinoma by regulating the miR-17-5p/acyl-CoA oxidase 1 axis.

Hai-xia Zhou; Lu-yao Wang; Shuai Chen; Dan-dan Wang; Zheng Fang

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circ_0005379 inhibits the progression of oral squamous cell carcinoma by regulating the miR-17-5p/acyl-CoA oxidase 1 axis.

Author: Hai-Xia ZHOU ¹ ; Lu-Yao WANG ¹ ; Shuai CHEN ¹ ; Dan-Dan WANG ¹ ; Zheng FANG ¹
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1. Center of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Publication Type:Journal Article
Keywords: acyl-CoA oxidase 1; apoptosis; circ_0005379; invasion; miR-17-5p; migration; oral squamous cell carcinoma; proliferation
MeSH: Acyl-CoA Oxidase; Animals; Carcinoma, Squamous Cell/genetics*; Cell Proliferation; Head and Neck Neoplasms; Humans; Mice; Mice, Nude; MicroRNAs; Mouth Neoplasms/genetics*; RNA, Circular; Squamous Cell Carcinoma of Head and Neck
From: West China Journal of Stomatology 2021;39(4):425-433
CountryChina
Language:English
Abstract: OBJECTIVES:To investigate the effects of circ_0005379 on the proliferation, apoptosis, migration, and invasion of oral squamous cell carcinoma (OSCC) cells and its mechanism.
METHODS:Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression levels of circ_0005379 and miR-17-5p in OSCC tissues and SCC15 cell lines. Western blot was used to detect the expression levels of acyl-CoA oxidase 1 (ACOX1). The circ_0005379 overexpression vector was transfected into SCC15 cells. Methyl thiazolyl tetrazolium blue staining, flow cytometry, Transwell, and Western blot were used to detect the effects of circ_0005379 overexpression on the proliferation, apoptosis, migration, and invasion of SCC15 cells and the expression of E-cadherin, β-catenin, and Snail proteins. Dual luciferase reporter assay and RNA immunoprecipitation were used to examine the regulation of circ_0005379, miR-17-5p, miR-17-5p, and ACOX1 in SCC15 cells. A nude mouse xenograft model of SCC15 cells stably overexpressing circ_0005379 was established, and the effect of circ_0005379 overexpression on the growth of xenografts in nude mice was observed.
RESULTS:Compared with adjacent cancer tissues, the expression levels of circ_0005379 and ACOX1 proteins in OSCC tissues were decreased (
CONCLUSIONS:circ_0005379 may inhibit the proliferation, migration, and invasion of OSCC cells by downregulating the expression of miR-17-5p and upregulating ACOX1, which promote apoptosis and inhibit tumor growth