Rapid Internal Control Reference Recombinase-Aided Amplification Assays for EBV and CMV Detection.

Yuan Gao; Yan Qing Tie; Lin Qing Zhao; He Tan; Nan Ding; Ya Xin Ding; Qi Guo; Rui Qing Zhang; Jin Rong Wang; Zi Wei Chen; Guo Hao Fan; Xin Xin Shen; Zhi Shan Feng; Xue Jun MA

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Rapid Internal Control Reference Recombinase-Aided Amplification Assays for EBV and CMV Detection.

Author: Yuan GAO ^{1
,

2
,

3} ; Yan Qing TIE ³ ; Lin Qing ZHAO ⁴ ; He TAN ^{1
,

3} ; Nan DING ³ ; Ya Xin DING ⁴ ; Qi GUO ⁴ ; Rui Qing ZHANG ⁵ ; Jin Rong WANG ^{1
,

5} ; Zi Wei CHEN ^{2
,

6} ; Guo Hao FAN ⁵ ; Xin Xin SHEN ⁵ ; Zhi Shan FENG ³ ; Xue Jun MA ^{2
,

7}
Author Information

1. Hebei Medical University, Shijiazhuang 050031, Hebei, China
2. NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
3. Hebei General Hospital, Shijiazhuang 050070, Hebei, China.
4. Laboratory of Virology, Beijing Key Laboratory of Etiology of Viral Diseases in Children, Capital Institute of Pediatrics, Beijing 100020, China.
5. NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
6. The Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, China.
7. Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, Hubei China.
Publication Type:Letter
Keywords: Cytomegalovirus; Epstein-Barr virus; Isothermal nucleic acid detection; Recombinase-aided amplification
MeSH: Adolescent; Adult; Child; Child, Preschool; Cytomegalovirus/genetics*; Cytomegalovirus Infections/virology*; DNA, Viral/analysis*; Epstein-Barr Virus Infections/virology*; Female; Herpesvirus 4, Human/genetics*; Humans; Infant; Infant, Newborn; Male; Middle Aged; Nucleic Acid Amplification Techniques; Recombinases/genetics*; Young Adult
From: Biomedical and Environmental Sciences 2021;34(8):650-655
CountryChina
Language:English
Abstract: Epstein-Barr virus (EBV) and cytomegalovirus (CMV), two of the most prevalent human herpesviruses, cause a wide spectrum of diseases and symptoms and are associated with serious health problem. In this study, we developed an internal control reference recombinase-aided amplification (ICR-RAA) assay for the rapid detection of EBV and CMV within 30 min. The assay had a sensitivity of 5 and 1 copies/test for EBV and CMV, respectively, with no cross reaction with other pathogens. In comparison with those of the commercial quantitative polymerase chain reaction (qPCR), the sensitivity of the EBV and CMV ICR-RAAs using extracted DNA was 93.33% and 84.84%, respectively; the specificity was 98.75% and 100.00%, respectively; and the Kappa values were 0.930 and 0.892 (