Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation.

Rong Jia; Ya Xiong Chen; Ya Rong DU; Bu Rong HU

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Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation.

Author: Rong JIA ^{1
,

2} ; Ya Xiong CHEN ³ ; Ya Rong DU ³ ; Bu Rong HU ^{1
,

4
,

5}
Author Information

1. Key Laboratory of Space Radiobiology of Gansu Province & CAS Key Laboratory of Heavy Ion Radiation Biology and Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China
2. School of Nuclear Science and Technology, University of Chinese Academy of Sciences, Beijing 100039, China.
3. Key Laboratory of Space Radiobiology of Gansu Province & CAS Key Laboratory of Heavy Ion Radiation Biology and Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China.
4. School of Nuclear Science and Technology, University of Chinese Academy of Sciences, Beijing 100039, China
5. Department of Radiation Medicine, School of Public Health and Management, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China.
Publication Type:Journal Article
Keywords: Cytokines; Dose estimation model; Low or high LET irradiation; MSD assay; Mouse plasma
MeSH: Animals; Biological Assay; Biomarkers/blood*; Carbon; Cytokines/blood*; Female; Heavy Ions; Linear Energy Transfer; Linear Models; Mice; Radiation Dosage; Radiation, Ionizing
From: Biomedical and Environmental Sciences 2021;34(7):540-551
CountryChina
Language:English
Abstract: Objective:To obtain precise data on the changes in the levels of 29 cytokines in mice after high or low linear energy transfer (LET) irradiation and to develop an accurate model of radiation exposure based on the cytokine levels after irradiation.
Methods:Plasma samples harvested from mice at different time points after carbon-ion or X-ray irradiation were analyzed using meso-scale discovery (MSD), a high-throughput and sensitive electrochemiluminescence measurement technique. Dose estimation equations were set up using multiple linear regression analysis.
Results:The relative levels of IL-6 at 1 h, IL-5 and IL-6 at 24 h, and IL-5, IL-6 and IL-15 at 7 d after irradiation with two intensities increased dose-dependently. The minimum measured levels of IL-5, IL-6 and IL-15 were up to 4.0076 pg/mL, 16.4538 pg/mL and 0.4150 pg/mL, respectively. In addition, dose estimation models were established and verified.
Conclusions:The MSD assay can provide more accurate data regarding the changes in the levels of the cytokines IL-5, IL-6 and IL-15. These cytokines could meet the essential criteria for radiosensitive biomarkers and can be used as radiation indicators. Our prediction models can conveniently and accurately estimate the exposure dose in irradiated organism.