Generation and phenotypic characterization of S100A9 gene knockout mice by CRISPR/Cas9-mediated gene targeting.
- Author:
Pei YAN
1
;
Da-Yan LIANG
1
;
Wen-Hao XU
1
;
Lu XUE
1
;
Meng-Fei YU
1
;
Jin-Hua SHEN
1
;
Qing-Hua LIU
1
;
Yong-Bo PENG
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bronchoalveolar Lavage Fluid; CRISPR-Cas Systems/genetics*; Calgranulin B; Disease Models, Animal; Gene Knockout Techniques; Gene Targeting; Lung; Mice; Mice, Inbred C57BL; Mice, Knockout; Ovalbumin; Phenotype
- From: Acta Physiologica Sinica 2021;73(3):482-490
- CountryChina
- Language:Chinese
- Abstract: S100 calcium binding protein A9 (S100A9) is involved in a variety of biological processes such as inflammation and tumor cell migration and invasion regulation. The purpose of this study was to construct S100A9 gene-edited mice by using CRISPR/Cas9 technology, thereby providing an animal model for exploring the biological functions of this gene. According to the S100A9 gene sequence, the single-stranded small guide RNA (sgRNA) targeting exons 2 and 3 was transcribed in vitro, and a mixture of Cas9 mRNA and candidate sgRNA was injected into mouse fertilized eggs by microinjection. Early embryos were obtained and transferred to surrogate mice, and F
