Study on the stability of SARS-CoV-2 at different temperatures
- VernacularTitle:新型冠状病毒温度稳定性研究
- Author:
Xiao-xian CUI
1
;
Jia-bin MOU
1
;
Zheng TENG
1
;
Yan-qiu ZHOU
1
;
Fang-hao FANG
1
;
Hong-you CHEN
1
;
Hui JIANG
1
;
Chong-shan LI
1
;
Min CHEN
1
;
Xi ZHANG
1
Author Information
- Publication Type:Research Article
- Keywords: SARS-CoV-2; stability; infectious titer; temperature
- From: Shanghai Journal of Preventive Medicine 2021;33(9):818-823
- CountryChina
- Language:Chinese
- Abstract: Objective:To observe the stability of severe acute respiratory syrdrome coronavirus （SARS-CoV-2） in cell cultures at different temperatures so as to provide basic data and scientific basis for the research and control of COVID-19 epidemic. Methods:The Vero E6 cells inoculated with SARS-CoV-2. According to TCID50， SARS-CoV-2 with different dilution （10-1， 10-3， 10-5， 10-6）were stored at 37 °C， 22.5 °C， and 4 °C for one to seven days， and then infectious titer was determined by micro cytopathogenic effect assay， observing cytopathic effect （CPE）， and real-time fluorescence quantitative testing. Results:SARS-CoV-2 was stable under 4 °C. The infectivity of high concentration （10-1 dilution） under 22.5 °C for seven days gradually decreased， while lower concentration completely lost infectivity after one day. The virus lost infectivity when stored at 37 °C for more than one day. Conclusion:SARS-CoV-2 is highly stable at 4 °C， sensitive to heat， and related to virus concentration.
