Application of DNA barcoding technology to national drug sampling inspection
10.16438/j.0513-4870.2021-0089
- VernacularTitle:DNA条形码技术在国家药品抽验中的应用研究
- Author:
Tian-yi XIN
1
;
Hai-xia YAN
2
;
Ran-jun LI
1
,
3
;
Qian LOU
1
;
Li-jun HAO
1
;
Bao-sheng LIAO
4
;
Ying LIU
2
;
Jing CHEN
2
;
You-gen CHEN
2
;
Xiao-wei DU
2
;
Hong-zhu GUO
2
;
Xin-tong FU
2
;
Jing-yuan SONG
1
,
5
Author Information
1. Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
2. Beijing Institute for Drug Control, NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine (Traditional Chinese Patent Medicine), Beijing Key Laboratory of Analysis and Evaluation on Chinese Medicine, Beijing 102206, China
3. School of Life and Science, Southwest Jiaotong University, Chengdu 610031, China
4. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
5. Engineering Research Center of Chinese Medicine Resource, Ministry of Education, Beijing 100193, China
- Publication Type:Research Article
- Keywords:
national drug sampling inspection;
DNA barcoding;
ITS2;
italic>COI;
market supervision
- From:
Acta Pharmaceutica Sinica
2021;56(5):1497-1508
- CountryChina
- Language:Chinese
-
Abstract:
Adulterants and counterfeits were found in some of the commercial traditional Chinese medicine (TCM) decoctions in Hongjin Xiaojie Jiaonang, Hongjin Xiaojie Pian, and Chaihuang Keli during the national drug sampling inspection. However, it was difficult to determine the species of the adulterants and counterfeits by conventional testing methods. Therefore, a total of 184 samples of the TCM decoctions and raw materials belong to the prescriptions of above mentioned traditional Chinese patent medicines, including Bupleuri Radix, Bajiaolian, Heimayi, and Shufuchong, were collected and authenticated by DNA barcoding technology. 111 ITS2 sequences were obtained from 115 commercial TCM decoctions and raw materials of Bupleuri Radix, among which 71 were Bupleurum chinense, three were B. scorzonerifolium, and 31 were closely related species in the same genus. In addition, counterfeits derived from different genera, such as Ailanthus altissima (one sample), Saposhnikovia divaricate (two samples), and Solidago decurrens (three samples), were also detected. 21 ITS2 sequences were obtained from 22 commercial TCM raw materials of Bajiaolian, among which 15 were Diphylleia sinensis and six were Dysosma versipellis and other species in genus Dysosma. For 22 Heimayi samples, PCR amplification of COI sequence was failed due to genomic DNA degradation. Among 38 Shufuchong samples, 24 COI sequences were obtained and only nine of them were the genuine species (Armadillidium vulgare) recorded in the Chinese Pharmacopoeia, 11 were Porcellio laevis, two were Mongoloniscus sinensis, and two samples could not be identified due to the limitation of database. This study demonstrates that DNA barcoding technology is suitable for the species authentication of the decoctions of traditional Chinese patent medicine prescription. It is a conductive way for the establishment of traceability system for the whole TCM industrial chain.
