Identification and expression analysis of the C2H2 gene family in Cannabis sativa L.
10.16438/j.0513-4870.2020-1902
- VernacularTitle:药用植物大麻C2H2基因家族鉴定与表达分析
- Author:
Mei-qi LIU
1
;
Wei SUN
2
;
Xiang-xiao MENG
2
;
Hui-hua WAN
2
;
Ting-xia LIU
1
;
Jia-ying SUN
1
;
Zhen WANG
1
;
Yao-lei MI
2
;
Wei MA
1
Author Information
1. College of Pharmaceutical Sciences, Heilongjiang University of Chinese Medicine, Harbin 150040, China
2. Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
italic>Cannabis sativa;
italic>C2H2 gene family;
bioinformatics;
phylogeny;
functional prediction
- From:
Acta Pharmaceutica Sinica
2021;56(5):1486-1496
- CountryChina
- Language:Chinese
-
Abstract:
C2H2 transcription factors play an important role in plant growth, development and the regulation of secondary metabolism. This article identifies members of the C2H2 gene family in Cannabis sativa L. at the genome level. Chromosomal location and linkage, evolutionary relationships, and identification of conserved motifs was determined from the C. sativa genome and transcriptome data using bioinformatics tools and on-line websites such as TBtools, MEGA software, NCBI, PlantTFDB, ExPASy, HMMSCAN, MEME, WoLFPSORT and PlantCARE. The results show that C. sativa contains 30 members of the C2H2 gene family (named CsC2H2-1-CsC2H2-30) distributed on 9 chromosomes. The encoded proteins range in length from 138 to 635 amino acids, and the theoretical isoelectric points range from 5.85 to 9.52. Molecular weights range from 15 909.48 to 68 445.53 Da. Transcriptome analysis showed that CsC2H2 was differentially expressed in the female flowers, bracts, leaves, and stems of the Diku variety and female flowers of nine different varieties of C. sativa. Quantitative real-time PCR verified that CsC2H2-1, CsC2H2-5, and CsC2H2-19 were significantly expressed in the female flowers and bracts of the Diku variety. This provides a theoretical basis for in-depth study of the function of the C2H2 gene family and the breeding of high-quality C. sativa varieties.
