Methyl jasmonate stimulates the synthesis and accumulation of asperosaponin VI in the roots of Dipsacus asper
10.16438/j.0513-4870.2021-0375
- VernacularTitle:茉莉酸甲酯对川续断根中川续断皂苷Ⅵ合成积累的影响
- Author:
Zheng-ping HU
1
;
Jiao XU
1
;
Tao ZHOU
1
;
Wei-ke JIANG
1
;
Juan GUO
2
;
Cheng-hong XIAO
1
;
Jin-qiang ZHANG
1
;
Bing-qing ZHOU
1
Author Information
1. Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
2. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
methyl jasmonate;
italic>Dipsacus asper;
asperosaponin VI;
ey enzyme gene;
expression level
- From:
Acta Pharmaceutica Sinica
2021;56(8):2302-2307
- CountryChina
- Language:Chinese
-
Abstract:
We investigated the effect of methyl jasmonate (MeJA) on the content of asperosaponin VI and the expression of genes involved in its synthesis. Dipsacus aspero seedlings were treated with MeJA at different concentrations of 50, 100, 150, 200 and 300 μmol·L-1, and leaves and roots were sampled following treatment for 1, 3 and 5 days. The content of asperosaponin VI and superoxide anion in the roots, malondialdehyde (MDA) content in leaves and superoxide dismutase were determined. The results show that 150 μmol·L-1 MeJA significantly increased the accumulation of asperosaponin VI in roots. The content of asperosaponin VI was greatest after treatment for 3 days, and was 2.16 times higher than the control. After MeJA treatment, SOD activity decreased and MDA content increased in leaves. Moreover, superoxide anion content in roots increased. The expression of squalene epoxidase (DaSE1) and geranyl diphosphate synthase (DaGPS), key enzymes in the synthesis of asperosaponin VI, were up-regulated compared with the control group. These results indicate that an optimal concentration of 150 μmol·L-1 MeJA increases the accumulation of asperosaponin VI by up-regulating the expression of key enzymes involved in the synthesis of asperosaponin VI, which facilitates resistance to adversity stress stimulated by MeJA.
