The effect of acute hypobaric hypoxia on bile acid composition in the small intestine of rats
10.16438/j.0513-4870.2021-0200
- VernacularTitle:急进高原对大鼠小肠中胆汁酸组成的影响
- Author:
Ya-ting ZHANG
1
,
2
;
Long-ji HUANG
1
,
2
;
An-peng ZHAO
3
;
Yue-mei SUN
3
;
Wen-bin LI
3
;
Juan-hong ZHANG
3
,
4
;
Rong WANG
1
,
2
Author Information
1. School of Pharmacy, Lanzhou University, Lanzhou 730000, China
2. Department of Pharmacy, the 940th Hospital of Joint Logistics Support Force, PLA, Lanzhou 730050, China
3. Department of Pharmacy, the 940th Hospital of Joint Logistics Support Force, PLA, Lanzhou 730050, China
4. College of Life Science, Northwest Normal University, Lanzhou 730030, China
- Publication Type:Research Article
- Keywords:
bile acid;
UHPLC-MS/MS;
acute hypobaric hypoxia;
metabolomics;
multivariate statistical analysis
- From:
Acta Pharmaceutica Sinica
2021;56(8):2260-2265
- CountryChina
- Language:Chinese
-
Abstract:
In order to clarify the influence of acute hypobaric hypoxia on the bile acids of the rat small intestine, we used ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to identify bile acids in the contents of the small intestine from untreated and acute hypobaric hypoxia-treated rats. Thirty-nine bile acids were detected; PCA and OPLS-DA analysis revealed marked differences in the composition of bile acids between the untreated and the acute hypobaric hypoxia groups. Bile acids were screened with VIP > 1, |log2FC| ≥ 1, P < 0.05, and a total of 7 bile acids with significant differences in content between the two groups were obtained, including 5 conjugated bile acids, 2 unconjugated bile acids; in addition, the content of conjugated bile acids has risen in the treated group. This study demonstrated the influence of high-altitude hypoxic environment on bile acid composition and metabolism in rats. All the animal experiments in this study were approved by the 940th Hospital Ethics Committee (approval No: 2020KYLL012).
