Paeoniae Rubra Radix decreases the hepatotoxicity of Psoraleae Fructus in an immunologically stressed rat model: a metabolic network analysis
10.16438/j.0513-4870.2021-0255
- VernacularTitle:免疫应激介导的赤芍缓解补骨脂肝脏毒性作用评价及其调控代谢网络分析
- Author:
Wen-xing WU
1
;
Sheng GUO
1
;
Li-ping WU
1
;
Ling XIA
1
;
Ming ZHAO
1
;
Quan LI
2
;
Heng-bin WANG
2
;
Jin-ao DUAN
1
Author Information
1. National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210023, China
2. Leiyunshang Pharmaceutical Co. Limited, Suzhou 215003, China
- Publication Type:Research Article
- Keywords:
Psoraleae Fructus;
Paeoniae Rubra Radix;
idiosyncratic hepatotoxicity;
metabolomics
- From:
Acta Pharmaceutica Sinica
2021;56(7):1789-1796
- CountryChina
- Language:Chinese
-
Abstract:
An immunologically stressed rat model was used in a metabolomics study on the ability of Paeoniae Rubra Radix to reduce the liver toxicity of Psoraleae Fructus. Different groups of rats were given the extracts of Psoraleae Fructus and Psoraleae Fructus together with Paeoniae Rubra Radix or combined with a non-toxic dose of lipopolysaccharide (LPS). The biochemical indices of liver function and pathological changes in liver tissue were used to evaluate histopathological changes. UHPLC-QTOF/MS was used to analyze the metabolic profile of serum samples, combined with principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) methods. The HMDB database and Metabo Analyst online tool were used for biomarker identification and metabolic pathway-enrichment analysis. The results show that the co-treatment Psoraleae Fructus and LPS resulted in significant liver injury, indicated by the elevation of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities, as well as obvious pathological changes. Liver injury was significantly decreased by treatment with Paeoniae Rubra Radix. Metabolomic analysis showed that the addition of Paeoniae Rubra Radix ameliorated the abnormal serum metabolism in rats mainly through regulation of arachidonic acid metabolism and glycerophospholipid metabolism pathways.
