Transcriptome analysis and exploration of genes involved in the biosynthesis of iridoids in Gentiana crassicaulis (Gentianaceae)
10.16438/j.0513-4870.2020-1921
- VernacularTitle:粗茎秦艽转录组及其环烯醚萜类生物合成相关基因分析与验证
- Author:
Heng KANG
1
;
Zhi-li ZHAO
1
;
Liang-hong NI
1
;
Wei-tao LI
1
;
Shu-juan ZHAO
1
;
Tong-hua LIU
2
,
3
Author Information
1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Tibetan Traditional Medical College, Lhasa 850000, China
3. Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
italic>Gentiana crassicaulis;
transcriptome;
iridoids;
biosynthesis;
metabolism pathway;
qRT-PCR
- From:
Acta Pharmaceutica Sinica
2021;56(7):2005-2014
- CountryChina
- Language:Chinese
-
Abstract:
italic>Gentiana crassicaulis Duthie ex Burk. in Gentiana (Sect. Cruciata), Gentianaceae, is one of the original plants of both Gentianae Macrophyllae Radix and Tibetan herb Jie-Ji Na-Bao, which contain such bioactive iridoids as gentiopicroside, loganic acid and others. In this study, based on previous work, the transcriptome of G. crassicaulis was sequenced and analyzed to construct transcriptome databases of roots, stems, leaves and flowers. qRT-PCR verification was conducted for parts of unigenes that may be key enzymes in the pathway of iridoid biosynthesis. The results are as follows: ① a total of 159 534 unigenes were obtained, with an average length of 679 bp. According to the functional classification of GO, unigenes can be divided into 3 categories with 67 branches. The unigenes were aligned in the KOG database and were classified into 25 categories according to function. ② In the KEGG database, 215 unigenes were implicated in 20 standard secondary metabolism pathways. The analysis shows that 305 unigenes encoded 28 key enzymes in the pathway of iridoid biosynthesis, and their expression in different organs is different; and ③ qRT-PCR was approximately consistent with RNA-Seq results. The 7 annotated unigenes identified in this study, HMGS, DXS, MCS, GPPS, G10H, 7-DLNGT and STR, all had higher relative expression levels in the above-ground parts (stem, leaf and flower) than in the underground part (root). Iridoids are common active and index components of such traditional Chinese medicines as Qinjiao, Longdan, Dangyao, and Qingyedan, among others. Therefore, this work provides basic scientific data for further development including obtaining active components or intermediates through biotechnology, exploring the accumulation of effective components, evaluating the quality of different ecotype varieties, and identifying authentic biosynthesis pathways of medicinal materials.
