The protective effect of drug-containing serums and polar extracts of Erzhi Wan on H2O2-induced hepatocyte senescence
10.16438/j.0513-4870.2021-0071
- VernacularTitle:二至丸及不同极性部位含药血清对H2O2诱导的肝细胞衰老的保护作用及物质基础研究
- Author:
Li FENG
;
Yi-fei WANG
;
Meng-ting GAO
;
Xin LI
;
Wei-feng YAO
;
Li ZHANG
- Publication Type:Research Article
- Keywords:
Erzhi Wan;
ifferent solvent extract;
hepatocyte senescence;
chemical constituent;
molecular docking
- From:
Acta Pharmaceutica Sinica
2021;56(4):1137-1146
- CountryChina
- Language:Chinese
-
Abstract:
Using a H2O2-induced BRL cell senescence model, we investigated the anti-aging effects of drug-containing serums of Erzhi Wan (EZW) and various polar extracts (petroleum ether, ethyl acetate, n-butanol, water, and iridoid glycoside-enriched fractions). Cell viability was detected by MTT assay. Cell senescence was evaluated with β-galactosidase staining assay. Intracellular reactive oxygen species (ROS) were measured by flow cytometry. UFLC-Q-TOF-MS/MS was used to identify chemical components in EZW and the extracts, and molecular docking technology was used to predict the anti-aging components of EZW. Results showed that treatment of cells with 600 μmol·L-1 H2O2 for 72 h markedly induced cell senescence, inhibited cell proliferation and increased intracellular β-galactosidase activity and ROS levels. If cells were pretreated with drug-containing serum of EZW this induction of senescence was decreased. A total of 49 chemical compounds were identified in EZW by liquid chromatography-mass spectrometry, 14 of these were identified by molecular docking as potential active ingredients. Based on these analyses, and the in vitro experiments with polar extracts, we conclude that the anti-aging components of EZW are triterpenes, flavonoids and phenyl alcohols, providing a basis for further elucidation of the active agents and mechanism of the anti-aging effect of EZW.
