Ginkgo biloba extract ameliorates streptozotocin-induced diabetes in rats as measured by non-targeted metabolomics
10.16438/j.0513-4870.2021-0064
- VernacularTitle:非靶向血液代谢组学研究糖尿病大鼠发生发展及银杏叶提取物干预的作用机制
- Author:
Fan ZHANG
1
;
Xun-xiu HU
1
;
Ding-xiang LI
1
;
Ran ZHANG
1
;
Lin-lin ZHAO
1
;
Yan DU
1
;
Dao-quan TANG
1
,
2
Author Information
1. Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, China
2. Department of Pharmaceutical Analysis, Xuzhou Medical University, Xuzhou 221004, China
- Publication Type:Research Article
- Keywords:
iabetes mellitus;
metabolomics;
plasma;
italic>Ginkgo biloba leaves extract;
LC-MS
- From:
Acta Pharmaceutica Sinica
2021;56(4):1127-1136
- CountryChina
- Language:Chinese
-
Abstract:
Metabolomics based on liquid chromatography coupled with mass spectrometry (LC-MS) was used to study the initiation and development of diabetes in rats, and the ability of Ginkgo biloba extract (GBE) to ameliorate this pathology. Diabetes mellitus (DM) was induced by intra-peritoneal injection of streptozotocin. The rats were randomly divided into a normal control group treated with drug-free solution (NC), a normal control group treated with GBE (N-GBE), a DM group treated with drug-free solution (DM), and a DM group treated with GBE (D-GBE); rats were maintained on this protocol for 9 weeks. Rat plasma was collected from the sixth week to the ninth week and then analyzed with LC-MS. Animal experimentation was approved by the Committee on the Ethics of Animal Experiments of Xuzhou Medical University. Twelve plasma metabolites with continuous differentiation were monitored to indicate dysfunction of metabolic pathways including fatty acid metabolism, phospholipid metabolism, amino acid metabolism, tricarboxylic acid cycle activity, bile acid metabolism, and purine metabolism to confirm the occurrence and development of DM. Treatment with GBE partially reversed the changes seen in five metabolites in DM rats, indicating that GBE could prevent the occurrence and development of DM by acting on fatty acid metabolism, phospholipid metabolism, amino acid metabolism, and the tricarboxylic acid cycle.
