Analysis of triterpenoic acids in different medicinal parts of Poria cocos (Schw.) Wolf using supercritical fluid chromatography
10.16438/j.0513-4870.2021-0045
- VernacularTitle:超临界流体色谱法分析茯苓不同药用部位中三萜酸类成分
- Author:
Na LI
1
,
2
;
Yuan-gui YANG
1
;
Yue CHEN
1
;
Rui XU
1
;
Li-hua GU
1
;
Yuan-biao XIE
3
;
Song-ming LI
3
;
Chang-sen ZHAN
3
;
Zheng-tao WANG
1
;
Li YANG
1
,
2
Author Information
1. The MOE Key Laboratory for Standardization of Chinese Medicines and SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
3. Shanghai Hutchison Pharmaceuticals Co., Ltd., Shanghai 201401, China
- Publication Type:Research Article
- Keywords:
italic>Poria cocos;
ifferent medicinal part;
triterpenoid acid;
ultra-performance convergence chromatography;
assay determination
- From:
Acta Pharmaceutica Sinica
2021;56(4):1120-1126
- CountryChina
- Language:Chinese
-
Abstract:
Qualitative and quantitative methods were used to establish the quality of different medicinal parts of Poria cocos (Poriae Cutis, rubra Poria, white Poria, Poria cum Radix Pini) by using ultra-performance convergence chromatography coupled with photo-diode array and quadrupole time-of-flight mass spectrometry (UPC2-PDA-Q-TOF/MSE). A total of 18 chromatographic peaks were detected from Poria cocos by UPC2-PDA. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used to compare the four medicinal parts. The results showed that there were significant differences in the components of different medicinal parts, and the main triterpenoic acids were poricoic acid A, poricoic acid B, dehydroeburicoic acid, and dehydrotrametenolic acid. When combined with the common active component polyporenic acid C, a method for determination of five triterpenoic acids in different parts of Poria cocos was established. These components could be separated within 15 min, and the amount of methanol was 3.63% of that of HPLC method. Taking the five triterpenoid acids as an index, the content of triterpenoid acids in different parts of Poria cocos from high to low were Poriae Cutis, rubra Poria, white Poria and Poria cum Radix Pini. The method is simple, rapid, and uses minimal solvent. The mobile phase of environment-friendly gas carbon dioxide has unique advantages in reducing environmental pollution, which can provide a basis for the development and standard formulation of Poria cocos and its related products.
