Determination of insulin lispro in rat plasma by LC-MS/MS and its application in a pharmacokinetics study
10.16438/j.0513-4870.2021-0670
- VernacularTitle:液相色谱-串联质谱法测定大鼠血浆中赖脯胰岛素及其药动学研究
- Author:
Xiao-chu SUN
1
,
2
;
Fei-fei LIN
3
;
Mi-mi WAN
3
;
Yue TONG
3
;
Lu CHANG
3
;
Meng YUAN
3
;
Ying-ying FENG
3
;
Guo-sheng TENG
1
;
Jia LIU
3
Author Information
1. School of Chemistry and Life Sciences, Changchun University of Technology, Changchun 130012, China
2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
3. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
- Publication Type:Research Article
- Keywords:
insulin lispro;
LC-MS/MS;
pharmacokinetics;
solid phase extraction
- From:
Acta Pharmaceutica Sinica
2021;56(9):2383-2388
- CountryChina
- Language:Chinese
-
Abstract:
Compared with human insulin, insulin lispro shows a faster hypoglycemic effect and a higher peak plasma concentration, which can better control postprandial hyperglycemia. In this study, we used a solid phase extraction pretreatment method and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to quantify insulin lispro in rat plasma. Bovine insulin was used as an internal standard. Plasma samples were separated on an ACQUITY UPLC Peptide CSH C18 column (2.1 mm × 50 mm, 1.7 μm) after solid phase extraction. Positive electrospray ionization was performed using multiple reaction monitoring (MRM) with transitions of m/z 1 162.5→217.2 for insulin lispro and m/z 1 157.5→136.0 for insulin bovine (internal standard). The method validation results showed that the linear range was 0.1 ng·mL-1 - 100 ng·mL-1; intra- and inter-day accuracy and precision met the acceptance criteria for biological sample analysis. The recovery of insulin lispro ranged from 63.1% to 68.1%. The method was applied in a pharmacokinetic study of insulin lispro following a single-dose subcutaneous administration to rats. Animal experiments were approved by the Experimental Animal Ethics Committee of Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
