Advancements in stabilization technologies for membrane protein and its application in drug screening
10.16438/j.0513-4870.2021-0573
- VernacularTitle:膜蛋白稳定技术及其在药物筛选中的应用进展
- Author:
Jia-hao FANG
;
Yu-hong CAO
;
Yu-zhen HE
;
Zhan-ying HONG
;
Yi-feng CHAI
- Publication Type:Research Article
- Keywords:
membrane protein;
stabilization technology;
etergent;
artificial membrane;
polymer;
lentivirus particles;
rug screening
- From:
Acta Pharmaceutica Sinica
2021;56(9):2325-2334
- CountryChina
- Language:Chinese
-
Abstract:
Membrane proteins are the main undertakers of biofilm function, and also the most important target group for innovative drug discovery and research. About 60% of drugs targets are membrane proteins. Due to the obvious aggregation and denaturation tendency of membrane proteins in aqueous solution, it is difficult to simulate the membrane like environment to maintain the correct conformation of membrane proteins in vitro, which results in the slower-growing research on the structure and function of membrane proteins and related ligand drugs than that of water-soluble proteins. Membrane protein stabilization technology is the premise of establishing high specificity, high sensitivity and high throughput drug screening methods for membrane protein ligands, which is of great significance. In this paper, some techniques for stable separation and purification of membrane proteins are reviewed, including detergents, artificial membranes, polymers, lentiviral particles and so on, as well as their specific applications in drug screening.
