Molecular markers based upon whole chloroplast genomes and identifying alpine Gentiana waltonii and G. lhassica (Gentianaceae)
10.16438/j.0513-4870.2021-0527
- VernacularTitle:基于叶绿体基因组的长梗秦艽、全萼秦艽物种DNA分子标记与鉴定
- Author:
Bo-ran DONG
1
;
Zhi-li ZHAO
1
;
Liang-hong NI
1
;
Dorje GAAWE
2
;
Tong-hua LIU
2
,
3
Author Information
1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Tibetan Traditional Medical College, Lhasa 850000, China
3. Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
Tibetan herb;
italic>Gentiana waltonii;
italic>Gentiana lhassica;
plastome;
molecular loci;
species-specific PCR assay
- From:
Acta Pharmaceutica Sinica
2021;56(9):2584-2591
- CountryChina
- Language:Chinese
-
Abstract:
As two original plants of Tibetan herb Jieji, Gentiana waltonii Burk. and Gentiana lhassica Burk. belong to Section Cruciata of Gentiana, Gentianaceae. Here, we report on whole chloroplast genome sequences in the alpine species, respectively, and the features of plastomes were investigated. The plastome of G. waltonii is 148 705 bp long (148 652 bp in G. lhassica) and encodes 112 genes, including 78 protein-coding genes, 30 transfer RNA genes, and 4 ribosomal RNA genes. Two pseudogenes, namely ψrps16 and ψinfA, were found in plastomes. In addition, two novel loci were detected, and a species-specific polymerase chain reaction assay was developed for differentiating G. waltonii and G. lhassica from 10 alpine species in Section Cruciata. Gentiana. Our study provides basic data for identifying Tibetan herbs, alpine species conservation and molecular phylogenetic studies of Gentiana and Gentianaceae.
