The mechanism by which Peganum harmala L. inhibits high glucose-induced tube formation in endothelial cells
10.16438/j.0513-4870.2021-0194
- VernacularTitle:骆驼蓬抑制高糖诱导的内皮细胞管道形成的作用机制研究
- Author:
Hong-li LI
1
;
Hui-hui SUN
1
;
Han-ying LIU
1
;
Qing-xuan JI
1
;
Jing-yun TIAN
2
;
Xing-zhuo SONG
2
;
Xi-rui WANG
2
;
Kun-xiu JIANG
2
;
Jing HAN
3
Author Information
1. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
2. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
3. Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
italic>Peganum harmala L.;
iabetic retinopathy;
retinal vascular endothelial cell;
tube formation;
network pharmacology
- From:
Acta Pharmaceutica Sinica
2021;56(9):2544-2552
- CountryChina
- Language:Chinese
-
Abstract:
The study investigates the mechanism by which Peganum harmala L. (Luotuopeng, LTP) inhibits tube formation in retinal vascular endothelial cells. Tube formation was induced by treatment of retinal vascular endothelial cells with glucose. The cells were divided into a normal group, model group, and an LTP group. The total length of tube formation was measured. The active components, targets, and pathway by which LTP acts in the treatment of diabetic retinopathy was explored by network pharmacology. The mRNA expression levels of targets [extracellular signal-regulated kinase 2 (ERK2), phosphoinositide 3 kinase catalytic alpha polypeptide (PIK3CA), serine/threonine-protein kinase 1 (AKT1)] related to the mitogen-activated protein kinase (MAPK) signaling pathway and vascular endothelial growth factor (VEGF) signaling pathway was measured by real-time PCR. The results of tube formation indicated that compared with the normal group, the total tube length increased in the model group (P < 0.01); after the treatment with LTP, the total tube length decreased compared with the model group (P < 0.01). Network pharmacology revealed that the targets of LTP included PIK3CA, AKT1, and ERK2, and the pathways involved the MAPK signaling pathway and the VEGF signaling pathway. Real-time PCR indicated that compared with the normal group, the mRNA expression levels of ERK2, PIK3CA and AKT1 were elevated in the model group (P < 0.05); after treatment with LTP, the mRNA expression levels of ERK2, PIK3CA and AKT1 decreased compared with the model group (P < 0.05). LTP may inhibit retinal vascular endothelial cell tube formation by regulating the MAPK signaling pathway and the VEGF signaling pathway. This study confirms the multi-targets and multi-pathways of LTP and provides a basis for its use in the treatment of diabetic retinopathy．
