Fingerprint Establishment of Rhizoma Ligustici from Different Origins and Their Chemometrics Analysis
- VernacularTitle:不同基原藁本药材的指纹图谱建立及其化学计量学分析
- Author:
Hui LIANG
1
;
Xiaojun PAN
1
;
Weisheng LYU
1
;
Wenhui YANG
1
;
Yuqin LUO
1
;
Liye PAN
1
;
Wenjie HUO
1
;
Zhenyu LI
1
Author Information
1. Guangdong Yifang Pharmaceutical Co.，Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule，Guangdong Foshan 528244，China
- Publication Type:Journal Article
- Keywords:
Ligusticum sinense;
Ligusticum jeholense;
Conioselinum vaginatium;
Origins identification;
UPLC;
Fingerprint
- From:
China Pharmacy
2021;32(19):2358-2362
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To establish UPLC fingerpri nt of Ligusticum sinense ，Ligusticum jeholense and Conioselinum vaginatium，and to conduct their chemometrics analysis so as to provide reference for the identification of Rhizoma Ligustici from different origins. METHODS ：UPLC method combined with Similarity Evaluation System of TCM Chromatographic Fingerprints （2012 edition） were used to establish the fingerprints of Rhizoma Ligustici from different origins. Chromatographic peak identification and similarity evaluation were carried out. Cluster analysis （CA），principal component analysis （PCA） and orthogonal partial least squares discriminant analysis （OPLS-DA）were performed to analyze Rhizoma Ligustici from different origins，and screen differential components. RESULTS ：Totally 13，11，11 characteristic peaks were identified in UPLC fingerprints of L. sinense ，L. jeholense and C. vaginatium ，respectively. Similarity evaluation showed that the similarity between C. vaginatium and L. jeholense were 0.312-0.541；that between C. vaginatium and L. sinense were 0.324-0.682；that between L. sinense and L. jeholense were 0.312-0.671，indicating there was great difference among Rhizoma Ligustici from different origins. CA，PCA and OPLS-DA showed that Rhizoma Ligustici from different origins were clustered into each category respectively ； chemical components represented by peak 10，peak 13，peak 12，peak 7 and peak 6 were differential components for Rhizoma Ligustici from 3 origins. CONCLUSIONS ：The study establishes UPLC fingerprint of Rhizoma Ligustici from different origins ， and screens 5 differential components ，which can be used to identify Rhizoma Ligustici from different origins.
