Content Determination of 17 Quality Markers in Dahuang Zhechong Pills
- VernacularTitle:大黄䗪虫丸中17种质量标志物的含量测定
- Author:
Chuankui FU
1
;
Kejia XU
1
;
Zimeng ZHANG
1
;
Yan HUANG
2
;
Zhipeng CHEN
1
;
Weidong LI
1
;
Li WU
1
Author Information
1. School of Pharmacy，Nanjing University of TCM，Nanjing 210023，China
2. Anhui Province Laboratory of Inflammation and Immune Mediated Diseases，Anhui Medical Universtiy，Hefei 230032，China
- Publication Type:Journal Article
- Keywords:
Dahuang zhechong pills;
Quality marker;
HPLC;
Quality stan dard;
Content determination
- From:
China Pharmacy
2021;32(19):2353-2357
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To establish the method for the content de termination of 17 quality markers in Dahuang zhechong pills（DHZCP）. METHODS ：HPLC method was adopted to determine the contents of 17 quality markers in 10 batches of DHZCP ， such as allantoin ，hypoxanthine，salidroside，hydroxypaeoniflorin，glycyrrhizin，isoglycyrrhizin，baicalin，p-methoxyphenylacetic acid，wogonin，cinnamic acid ，apigenin，naringin，norwogonin，aloe emodin ，rhein，chrysin，emodin. The determination was performed on Kromasil 100-5-C18（250 mm × 4.6 mm，5 μm）column with mobile phase consisted of 0.1％ phosphoric acid solution-acetonitrile （gradient elution ） at the flow rate of 1.0 mL/min. The column temperature was 30 ℃ ，the detection wavelength was 210 nm and the sample size was 20 μ L. RESULTS：The linear range of above 17 quality markers were 5.74-183.53，6.51-208.24，4.30-137.65，4.60-147.06，4.12-131.76，4.25-135.88，6.31-201.76，4.60-147.06，1.94-62.06，4.47- 142.94，0.69-22.06，2.29-73.24，2.33-74.41，1.42-45.29，6.65-212.94，1.11-35.44 and 1.47-47.06 μg/mL，respectively（all R2≥ 0.999 0）. RSDs of precision ，repeatability，stability and durability tests were all less than 2％（n＝6）；average recovery of 17 quality markers ranged from 96.31％ to 101.73％，and the RSDs were less than 3％（n＝6）. CONCLUSIONS ：The method is simple， rapid，speific，specise，reproducible，stable，accurate and durable ，and can be used for improving the quality standard of DHZCP.
