Formulation Optimization of Zuojin Pectin Capsule and Its Protective Effect on Gastric Ulcer Model Rats
- VernacularTitle:左金果胶胶囊的处方优化及其对胃溃疡模型大鼠的保护作用
- Author:
Guiqian YANG
1
;
Wen LIU
1
,
2
;
Ling TAO
1
;
Xiangchun SHEN
1
;
Shuoyao SONG
1
;
Huan ZHANG
1
;
Herong LI
1
;
Shouli WANG
1
;
Huiyun SHI
1
Author Information
1. School of Pharmacy，Guizhou Medical University/Engineering Center for the Highly Efficient Utilization of Natural Medicine Resources in Guizhou Province/Key Lab for Pharmacology and Pharmacogenesis Evaluation of Natural Medicine in Guizhou Universities/Guizhou Medical University-Guiyang Joint Key Laboratory/Key Laboratory for Optimal Utilization of Natural Medicine Resources，Guiyang 550025，China
2. The Affiliated Hospital of Guiz hou Medical University，Guiyang 550025，China
- Publication Type:Journal Article
- Keywords:
Zuojin pectin capsule;
Gastric ulcer;
Orthogonal test;
Sustained-release effect;
Rat
- From:
China Pharmacy
2021;32(19):2327-2335
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To optimize the form ulation of Zuojin pectin c apsules，and to prepare modern Zuojin pectin capsules with protective effects against gastric ulcers. METHODS ：The formulation of Zuojin pectin capsules was optimized with orthogonal test with the contents of pectin ，soluble starch and dextrin as factors ，using formability ，moisture absorption and flow ability as indicators. Zuojin pectin capsule was prepared by wet granulation filling method with Zuojin extract powder as raw material. The contents of palmatine hydrochloride ，berberine hydrochloride ，evodiamine and rutaecarpin were evaluated by HPLC. Basket method was used to investigate the release behavior of the capsule in 0.1 mol/L HCl solution. The gastric ulcer model of rats was established by intragastric administration of 75％ ethanol. Gastric ulcer index ，the inhibition rate of gastric ulcer and the pathological sections were used as indexes to investigate the protective effect of Zuojin pectin capsules （the doses were 54，108， 216 mg/kg）on gastric ulcer. RESULTS ：The optimal formulation of Zuojin pectin capsules included 45％ pectin，12％ soluble starch，27％ dextrin and 1％ xylitol. Results of in vitro drug ， release showed that palmatine hydrochloride and berberine， hydrochloride in Zuojin pectin capsules released 53.76％ and No.54.82％ respectively within 1 h，completely released at about 8 h， and conformed to the zero-order release behavior. 2492109374@qq.com Different doses of Zuojin pectin capsule could improve the ulcer injury of gastric tissue in gastric ulcer model rats to different extent ，and significantly reduced the gastric ulcer index（P＜0.01），significantly increased the inhibition rate of gastric ulcer and the percentage of positive expression area of Schiff ’s iodate staining （P＜0.01）. CONCLUSIONS ：Zuojin pectin capsule with protective effect on gastric ulcer and certain sustained- release effect is successfully prepared.
