Immunoregulatory effect of polysaccharides derived from chroogomphus rutilus on macrophage cell line RAW264.7
10.12206/j.issn.1006-0111.202102005
- VernacularTitle:血红铆钉菇多糖对RAW264.7巨噬细胞免疫调节作用研究
- Author:
Jiatai DOU
1
,
2
;
Youhao LIU
3
;
Qichao LIANG
4
;
Yiyan WU
4
Author Information
1. School of pharmacy, Mudanjiang Medical College, Mudanjiang 157011, China
2. The 78th Group Army Hospital of the PLA, Mudanjiang 157011, China.
3. Hongqi Hospital of Mudanjiang Medical College, Mudanjiang 157011, China.
4. School of pharmacy, Mudanjiang Medical College, Mudanjiang 157011, China.
- Publication Type:Researchreports
- Keywords:
CRPS25-Ⅱ;
RAW264.7 cells;
immune regulation
- From:
Journal of Pharmaceutical Practice
2021;39(5):449-453
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the immunomodulatory effect of polysaccharides (CRPS25-Ⅱ) derived from Chroogomphus rutilus on mouse mononuclear macrophages, RAW264.7 cells. Methods RAW264.7 cells were resuspended and cultured, cell suspension was prepared. The blank control group and CRPS25-Ⅱ groups with different mass concentrations (1, 20, 40, 80 and 160 μg/ml) were set up. MTT assay was used to determine the cytotoxicity of CRPS25-Ⅱ on RAW264.7 cells. RT-PCR was used to detect the effects of CRPS25-Ⅱ on the secretion of immune regulatory factors IL-6 and TNF-α from RAW264.7 cells. Western blot was used to detect the effects of CRPS25-Ⅱ on the expression of p-P65 protein in NF-κB pathway of RAW264.7 cells. Results The results showed that CRPS25-Ⅱ (1−160 μg/ml) had no obvious cytotoxicity. CRPS25-Ⅱ (1−160 μg/ml) increased the secretion of cytokines, and thus promoted the mRNA expression of IL-6 and TNF-α. CRPS25-Ⅱ increased the phosphorylation of p-P65 protein and activated the NF-κB signaling pathway, and thus promoted the immune regulation of cells. CRPS25-Ⅱ (1−160 μg/ml) could increase the p-P65 protein, and the promoting effects of CRPS25-Ⅱshowed an upward trend in the concentration range of 1−40 μg/ml and gradually weakened in the concentration range of 40−160 μg/ml. Conclusion Polysaccharides derived from chroogomphus rutilus had no cytotoxicity to mouse macrophages, and could promote the secretion of inflammatory factors IL-6 and TNF-α and activate the NF-κB signaling pathway, thus playing an immunomodulatory role.
