Quantitative determination of quercetin in Honghuixiang injection by HPLC
10.12206/j.issn.1006-0111.202103041
- VernacularTitle:用HPLC法测定红茴香注射液中槲皮苷的含量
- Author:
Cheng CHENG
1
;
Xiangmei CHEN
1
;
Jian MA
1
;
Chuan ZHAO
1
Author Information
1. Department of Pharmacy , Medical Security Centre, Chinese PLA General Hospital, Beijing 100048, China.
- Publication Type:Researchreports
- Keywords:
HPLC;
Honghuixiang injection;
quercetin
- From:
Journal of Pharmaceutical Practice
2021;39(5):447-448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish quantitative methods to assay quercetin in Honghuixiang injection by HPLC. Methods Dikma C18 column(250 mm×4.6 mm, 5 μm) was used for the assay with acetonitrile −0.1% phosphoric acid (25∶75) as the mobile phase. Flow rate was 1.0 ml/min. The column temperature was 30 ℃. The detection wavelength was at 256 nm. Results Quercetin showed good linear relationship within the range of 0.2150–3.225 μg. The correlation coefficient was 0.999 6. The average recovery was 99.39% with RSD 0.82% (n=6). The repeatability was 1.194 mg/ml with RSD 0.40%. Conclusion The average quercetin content in three batches of Honghuixiang injection was 1.191 mg/ml. This method is simple, rapid and accurate. It can be used for the determination of quercetin in Honghuixiang injection.
