Quantitative analysis of five compounds in gallnut lotion by UPLC-MS/MS
10.12206/j.issn.1006-0111.202102010
- VernacularTitle:UPLC-MS/MS法测定五倍子洗剂中5种指标性成分的含量
- Author:
Qun ZHANG
1
;
Yu CHEN
2
;
Xiaowei ZHI
2
;
Qiuping XU
2
;
Yingnan YANG
2
;
Liang ZHAO
2
;
Chengjian LI
2
Author Information
1. Shanghai Baoshan Aged-nursing Hospital, Shanghai 200431, China.
2. Luodian Hospital of Baoshan District, Shanghai 201908, China.
- Publication Type:Researchreports
- Keywords:
UPLC- MS/MS;
gallnut lotion;
determination
- From:
Journal of Pharmaceutical Practice
2021;39(5):437-441
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to simultaneously detect the contents of gallic acid, syringin, phellodendrine, aesculin and rhein in the gallnut lotion. Methods An UPLC- MS/MS method was established. Separation was performed on an Agilent Poroshell 120 EC-C18(2.1 mm×150 mm, 2.7 μm)with a gradient mobile phase system of 0.2% formic water-acetonitrile solution. The flow rate was 0.3 ml/min. The temperature of column was 30 ℃. The injection volume was 2 μl. The MS detection was in dynamic MRM mode. Results gallic acid, syringin, phellodendrine, aesculin and rhein were successfully separated using this method, with good linear relationship as the ranges of 153.8-15380、10.31-1031、5.265-526.5、50.70-5070、1.054-105.4 ng/ml, respectively. The precision, repeatability, stability and recovery were good. Conclusion This UPLC-MS/MS method is stable, rapid, and reproducible., It is suitable for detecting the contents of gallic acid, syringin, phellodendrine, esculetin and in the gallnut lotion.
