The Effects of Intravenous Ketamine on Neurologic Injury and Glutamate Receptor Gene Expression after Transient Spinal schemia in the Rat.
10.4097/kjae.2002.42.5.660
- Author:
Jae Young KWON
1
;
Young Chan JOO
;
Chul Hong KIM
;
Kyoung Hoon KIM
;
Hae Kyu KIM
;
Seong Wan BAIK
Author Information
1. Department of Anesthesiology, College of Medicine, Pusan National University, Busan, Korea. jykwon@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Ketamine;
glutamate receptor 5 mRNA;
N-methyl-D-asparatate receptor;
spinal cord ischemia
- MeSH:
Animals;
Extremities;
Gene Expression*;
Glutamic Acid*;
Ischemia;
Ketamine*;
Models, Theoretical;
Motor Neurons;
Neurons;
Rats*;
Receptors, Glutamate*;
RNA, Messenger;
Spinal Cord;
Spinal Cord Ischemia
- From:Korean Journal of Anesthesiology
2002;42(5):660-666
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Massive release of glutamate plays an important role in ischemic neuronal injury, and modification of this process may provide neuroprotection. We studied the protective effects of the N- methyl-D-aspartate receptor antagonist ketamine on hind limb motor function and glutamate receptor of gene expression in an experimental model of spinal cord ischemia. METHODS: Transient spinal cord ischemia was induced by 15 min of thoracic aortic occlusion in 24 anesthetized Sprague-Dawly rats. Rats were randomly assigned to one of three treatment groups (n = 8 each): C group, no intervention; K30 group, ketamine 30 mg/kg intravenously; or K50 group, ketamine 50 mg/kg intravenously. Normothermia (38degreesC) was maintained during ischemia. After spinal ischemia neurologic function was evaluated immediately and after 1, 2 and 3 hours. After 3 hours rats were euthanized and spinal cords were removed for the assay of NMDAR and mGluR5 mRNA. RESULTS: Neurologic outcome was better in the K30 group than the C or K50 group (P < 0.05). The NMDAR mRNA expression of the K30 and K50 group were greater than those of the C group. The mGluR5 mRNA expression increased after spinal ischemia. There were no differences between groups. CONCLUSIONS: In this study demonstrated that treatment with ketamine 30 mg/kg intravenously before ischemia increases tolerance of spinal cord motor neurons in a period of normothermic ischemia.
