Application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in the analysis of the pollution homology of clean room in workshop
10.3969/j.issn.1006-2483.2021.05.010
- VernacularTitle:基质辅助激光解吸电离飞行时间质谱在车间洁净室污染同源性分析中的应用
- Author:
Qiang DU
1
;
Ping YAO
1
;
Xujian YAO
1
;
Ying ZHAO
1
Author Information
1. Changzhou Center for Disease Control and Prevention, Changzhou , Jiangsu 213022 , China
- Publication Type:Journal Article
- Keywords:
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry;
Homology analysis;
Staphylococcus epidermidis
- From:
Journal of Public Health and Preventive Medicine
2021;32(5):43-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the source of microbial contamination in the clean room of the workshop. Methods Microbiological sampling was carried out from the air, environment and personnel of the workshop. The samples were cultivated, the microorganisms were detected by MALDI-TOF-MS, and homology analysis was performed with the microbial identification system of the instrument. Results A total of 14 species and 41 strains of bacteria were detected. Nine strains of Staphylococcus epidermidis were selected for homology analysis, and the Staphylococcus epidermidis from personnel gloves and headgear had 94% homology. There was 83% homology among the staphylococcus epidermidis derived from the sedimentation bacteria, the ground environment and personnel, which was higher than the 71% of the standard strain. Conclusion The homology analysis demonstrates that the pollution in the clean room of the workshop mainly comes from personnel, and secondly comes from the environment outside the workshop. Enterprises need to strengthen management to prevent the occurrence of microbial contamination. MALDI-TOF-MS can be used for rapid detection of complex environmental bacteria and for homology analysis.
