Drug-resistant gene polymorphisms in Plasmodium falciparum isolated from Bioko Island, Equatorial Guinea in 2018 and 2019
10.16250/j.32.1374.2021128
- VernacularTitle:2018—2019年赤道几内亚Bioko岛恶性疟原虫 抗药性基因多态性分析
- Author:
Jin-Quan HE
1
,
2
;
Jiang-Tao CHEN
3
,
4
;
Jing-He LI
3
,
5
;
Wei-Zhong CHEN
6
;
Xue-Yan LIANG
7
,
8
;
Hui-Ying HUANG
6
;
Hua-Gui WEI
9
;
Wei-Yi HUANG
9
;
Jun-Li WANG
9
;
Min LIN
6
,
10
;
Pei-Kui YANG
6
;
Xin-Yao CHEN
6
;
Xiang-Zhi LIU
6
Author Information
1. Department of Laboratory Medicine, Humen Hospital of Dongguan City, Guangdong Province, Dongguan 523000, China
2. The Chinese Medical Aid Team to the Republic of Equatorial Guinea, Guangdong Province, China
3. The Chinese Medical Aid Team to the Republic of Equatorial Guinea, Guangdong Province, China
4. Department of Laboratory Medicine, Huizhou Central Hospital, Guangdong Province, China
5. Department of Laboratory Medicine, Shijie Hospital, Dongguan City, Guangdong Province, China
6. Chaozhou People’s Hospital Affiliated to Shantou University, China
7. Department of Laboratory Medicine, Huizhou Central Hospital, Guangdong Province, China
8. Chaozhou People’s Hospital Affiliated to Shantou University, China
9. School of Laboratory Medicine, Youjiang Medical University for Nationalities, China
10. School of Laboratory Medicine, Youjiang Medical University for Nationalities, China
- Publication Type:Journal Article
- Keywords:
Plasmodium falciparum;
Drug resistance;
Genetic polymorphism;
Equatorial Guinea
- From:
Chinese Journal of Schistosomiasis Control
2021;33(4):396-400
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genetic polymorphisms of Plasmodium falciparum multidrug resistance protein 1 (PfMDR1), chloroquine resistance transporter (PfCRT) and Kelch 13 (PfK13) genes in Bioko Island, Equatorial Guinea, so as to provide insights into the development of the malaria control strategy in local areas. Methods A total of 85 peripheral blood samples were collected from patients with Plasmodium falciparum infections in Bioko Island, Equatorial Guinea in 2018 and 2019, and genomic DNA was extracted. The PfMDR1, PfCRT and PfK13 genes were amplified using a nested PCR assay. The amplification products were sequenced, and the gene sequences were aligned. Results There were no mutations associated with artemisinin resistance in PfK13 gene in Bioko Island, Equatorial Guinea, while drug-resistant mutations were detected in PfMDR1 and PfCRT genes, and the proportions of PfMDR1_N86Y, PfMDR1_Y184F and PfCRT_K76T mutations were 35.29% (30/85), 72.94% (62/85) and 24.71% (21/85), respectively. Conclusion There are mutations in PfMDR1, PfCRT and PfK13 genes in P. falciparum isolates from Bioko Island, Equatorial Guinea.
